Back to Search
Start Over
Viral growth assay to evaluate the replicative capacity of HIV-1 isolates
- Source :
- Journal of virological methods. 115(2)
- Publication Year :
- 2003
-
Abstract
- The replicative capacity of HIV is studied by carrying out replication-competition experiments with the insertion of the gene of interest. These assays cannot capture the complicated patterns of mutations of different genes.A cross sectional study was carried out on 10 HIV-infected nai;ve patients and on 15 patients failing HAART. The CD8-depleted PBMCs, with known proviral DNA and cellular HIV-RNA copy numbers, were cultured. A reference curve was determined using the data obtained from 10 nai;ve patients. The replicative capacity was calculated as the ratio multiplied by 100 of the p24 antigen level of isolates over the p24 antigen level determined on the reference curve.A linear correlation between p24 antigen level and the infectious doses of HIV-DNA alone or plus cellular RNA copy number of PBMCs was found in naive patients (r=0.63, P
- Subjects :
- Virus Cultivation
Settore MED/17 - Malattie Infettive
HIV Core Protein p24
HIV Infections
Drug resistance
Biology
Virus Replication
Peripheral blood mononuclear cell
Virus
Antiretroviral Therapy, Highly Active
Virology
Humans
Treatment Failure
Gene
Replicative capacity
RNA
HIV
biology.organism_classification
Viral growth assay
Reverse transcriptase
Viral replication
Lentivirus
DNA, Viral
HIV-1
RNA, Viral
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 115
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of virological methods
- Accession number :
- edsair.doi.dedup.....d12a26d25664e96bd1ae6c1ccbdd9921