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Synaptophysin: A reliable marker for medulloblastomas
- Source :
- Virchows Archiv A Pathological Anatomy and Histopathology. 411:53-59
- Publication Year :
- 1987
- Publisher :
- Springer Science and Business Media LLC, 1987.
-
Abstract
- Synaptophysin is an acidic, integral membrane glycoprotein (Mr 38,000) of presynaptic vesicles in various neurons and neuroendocrine cells, and in tumours derived from such cells. By indirect immunofluorescence microscopy of cryostat sections, using the monoclonal antibody SY 38 to synaptophysin, a consistent positive immunoreactivity was observed in all medulloblastomas (n = 6) and neuroblastomas (n = 3) as well as a ganglioneuroma and a glioneuronal hamartoma. The presence of synaptophysin in medulloblastomas was confirmed biochemically by immunoblotting experiments. For purpose of comparison, the expression of intermediate-sized filament (IF) proteins was also examined. While neurofilament proteins were consistently expressed in the neuroblastomas (3/3), the ganglioneuroma and the glioneuronal hamartoma, IF distribution in medulloblastomas was variable. A neurofilament-positive type of tumour (1/6) could be distinguished from vimentin-expressing neoplasms (4/6) by immunocytochemistry. These data indicate that synaptophysin is a reliable marker for medulloblastomas as well as other differentiated and undifferentiated neuronal tumours and in this respect is superior to the more heterogeneous expression patterns of IF proteins in these tumours.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Hamartoma
Immunocytochemistry
Synaptophysin
Fluorescent Antibody Technique
Vimentin
Pathology and Forensic Medicine
Neuroblastoma
Intermediate Filament Proteins
Neurofilament Proteins
medicine
Humans
Ganglioneuroma
Cerebellar Neoplasms
Child
Molecular Biology
Immunoassay
Medulloblastoma
biology
Infant
Membrane Proteins
Cell Biology
General Medicine
medicine.disease
stomatognathic diseases
Membrane protein
Child, Preschool
biology.protein
Electrophoresis, Polyacrylamide Gel
Subjects
Details
- ISSN :
- 14322307 and 01747398
- Volume :
- 411
- Database :
- OpenAIRE
- Journal :
- Virchows Archiv A Pathological Anatomy and Histopathology
- Accession number :
- edsair.doi.dedup.....d0fd45af47de10e83aa02015e8962dad