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A Novel Organic Selenium Compound Exerts Unique Regulation of Selenium Speciation, Selenogenome, and Selenoproteins in Broiler Chicks
- Source :
- The Journal of nutrition. 147(5)
- Publication Year :
- 2017
-
Abstract
- Background: A new organic selenium compound, 2-hydroxy-4-methylselenobutanoic acid (SeO), displayed a greater bioavailability than sodium selenite (SeNa) or seleno-yeast (SeY) in several species.Objective: This study sought to determine the regulation of the speciation of selenium, expression of selenogenome and selenocysteine biosynthesis and degradation-related genes, and production of selenoproteins by the 3 forms of selenium in the tissues of broiler chicks.Methods: Day-old male chicks (n = 6 cages/diet, 6 chicks/cage) were fed a selenium-deficient, corn and soy-based diet [base diet (BD), 0.05 mg Se/kg] or the BD + SeNa, SeY, or SeO at 0.2 mg Se/kg for 6 wk. Plasma, livers, and pectoral and thigh muscles were collected at weeks 3 and 6 to assay for total selenium, selenomethionine, selenocysteine, redox status, and selected genes, proteins, and enzymes.Results: Although both SeY and SeO produced greater concentrations (P < 0.05) of total selenium (20-172%) and of selenomethionine (≤15-fold) in the liver, pectoral muscle, and thigh than those of SeNa, SeO further raised (P < 0.05) these concentrations by 13-37% and 43-87%, respectively, compared with SeY. Compared with the BD, only SeO enhanced (P < 0.05) the mRNA of selenoprotein (Seleno) s and methionine sulfoxide reductase B1 (Msrb1) in the liver and thigh (62-98%) and thioredoxin reductase (TXRND) activity in the pectoral and thigh muscles (20-37%) at week 3. Furthermore, SeO increased (P < 0.05) the expression of glutathione peroxidase (Gpx) 3, GPX4, SELENOP, and SELENOU relative to the SeNa group by 26-207%, and the expression of Selenop, O-phosphoseryl-transfer RNA (tRNA):selenocysteinyl-tRNA synthase, GPX4, and SELENOP relative to the SeY group by 23-55% in various tissues.Conclusions: Compared with SeNa or SeY, SeO demonstrated a unique ability to enrich selenomethionine and total selenium depositions, to induce the early expression of Selenos and Mrsb1 mRNA and TXRND activity, and to enhance the protein production of GPX4, SELENOP, and SELENOU in the tissues of chicks.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Thioredoxin-Disulfide Reductase
Thioredoxin reductase
Medicine (miscellaneous)
chemistry.chemical_element
GPX4
Amino Acyl-tRNA Synthetases
03 medical and health sciences
chemistry.chemical_compound
Selenium
Sodium Selenite
Internal medicine
Yeasts
medicine
Animals
RNA, Messenger
Selenium Compounds
Selenomethionine
Selenoproteins
Selenium Compound
chemistry.chemical_classification
Glutathione Peroxidase
Nutrition and Dietetics
Selenocysteine
Glutathione peroxidase
Muscles
Broiler
Butyrates
030104 developmental biology
Endocrinology
chemistry
Liver
Environmental chemistry
Methionine Sulfoxide Reductases
Animal Nutritional Physiological Phenomena
Selenoprotein
Chickens
Subjects
Details
- ISSN :
- 15416100
- Volume :
- 147
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- The Journal of nutrition
- Accession number :
- edsair.doi.dedup.....d0e834ffd3a0ae60303f5c1d73b3395f