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Loss of endothelium-dependent relaxation in abdominal aorta preserved in a co-storage system
- Source :
- Transplant international : official journal of the European Society for Organ Transplantation. 17(11)
- Publication Year :
- 2003
-
Abstract
- The potentially detrimental influence of parenchymal cells on endothelial function during preservation in UW solution was examined by co-storage of rat abdominal aortic rings with isolated liver cells. Cold storage of rings in UW solution alone for up to 96 h had no effect on the response to acetylcholine, though constriction was progressively lost. Co-storage of rings with liver cells resulted in no loss of sodium nitroprusside response, but the relaxation response to acetylcholine was reduced. The loss of acetylcholine response could not be attributed to Kupffer cells, the lowering of pH, oxygen depletion, or the loss of constriction. A similar loss of endothelial function was observed in rings stored in pieces of liver, kidney or heart. We conclude that parenchymal cells exude factors during preservation by cold storage which reversibly inhibit vascular NO production. These factors could significantly impair whole organ function on reperfusion.
- Subjects :
- Male
Nitroprusside
medicine.medical_specialty
Adenosine
Time Factors
Endothelium
Kupffer Cells
Allopurinol
Vasodilator Agents
Organ Preservation Solutions
Cold storage
Vasodilation
In Vitro Techniques
Rats, Sprague-Dawley
Raffinose
Internal medicine
medicine
Animals
Insulin
Viaspan
Aorta, Abdominal
Cryopreservation
Transplantation
business.industry
Drug Synergism
Anatomy
Organ Preservation
Hydrogen-Ion Concentration
Glutathione
Acetylcholine
Rats
Endothelial stem cell
medicine.anatomical_structure
Endocrinology
Liver
Hepatocyte
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Circulatory system
Hepatocytes
Endothelium, Vascular
business
medicine.drug
Subjects
Details
- ISSN :
- 09340874
- Volume :
- 17
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Transplant international : official journal of the European Society for Organ Transplantation
- Accession number :
- edsair.doi.dedup.....d0e3d7638417b102aa3a8bba96896bb0