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Visualization of plasmid delivery to keratinocytes in mouse and human epidermis
- Source :
- Scientific Reports
- Publication Year :
- 2011
-
Abstract
- The accessibility of skin makes it an ideal target organ for nucleic acid-based therapeutics; however, effective patient-friendly delivery remains a major obstacle to clinical utility. A variety of limited and inefficient methods of delivering nucleic acids to keratinocytes have been demonstrated; further advances will require well-characterized reagents, rapid noninvasive assays of delivery, and well-developed skin model systems. Using intravital fluorescence and bioluminescence imaging and a standard set of reporter plasmids we demonstrate transfection of cells in mouse and human xenograft skin using intradermal injection and two microneedle array delivery systems. Reporter gene expression could be detected in individual keratinocytes, in real-time, in both mouse skin as well as human skin xenografts. These studies revealed that non-invasive intravital imaging can be used as a guide for developing gene delivery tools, establishing a benchmark for comparative testing of nucleic acid skin delivery technologies.
- Subjects :
- Keratinocytes
Microinjections
Green Fluorescent Proteins
Transplantation, Heterologous
Human skin
Gene delivery
Biology
Transfection
Article
03 medical and health sciences
Mice
0302 clinical medicine
Drug Delivery Systems
Genes, Reporter
medicine
Bioluminescence imaging
Animals
Humans
Luciferases
030304 developmental biology
0303 health sciences
Reporter gene
Multidisciplinary
integumentary system
Melanoma
Skin Transplantation
medicine.disease
Molecular biology
Recombinant Proteins
3. Good health
Cell biology
Microscopy, Fluorescence
Naked DNA
030220 oncology & carcinogenesis
Luminescent Measurements
Nucleic acid
Female
Epidermis
Plasmids
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....d0da69685de8ea0250b9f84b25675e71