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Epigenetic-based combinatorial resveratrol and pterostilbene alters DNA damage response by affecting SIRT1 and DNMT enzyme expression, including SIRT1-dependent γ-H2AX and telomerase regulation in triple-negative breast cancer
- Source :
- BMC Cancer
- Publication Year :
- 2015
- Publisher :
- BioMed Central, 2015.
-
Abstract
- Background Nutrition is believed to be a primary contributor in regulating gene expression by affecting epigenetic pathways such as DNA methylation and histone modification. Resveratrol and pterostilbene are phytoalexins produced by plants as part of their defense system. These two bioactive compounds when used alone have been shown to alter genetic and epigenetic profiles of tumor cells, but the concentrations employed in various studies often far exceed physiologically achievable doses. Triple-negative breast cancer (TNBC) is an often fatal condition that may be prevented or treated through novel dietary-based approaches. Methods HCC1806 and MDA-MB-157 breast cancer cells were used as TNBC cell lines in this study. MCF10A cells were used as control breast epithelial cells to determine the safety of this dietary regimen. CompuSyn software was used to determine the combination index (CI) for drug combinations. Results Combinatorial resveratrol and pterostilbene administered at close to physiologically relevant doses resulted in synergistic (CI
- Subjects :
- Genomic stability
Telomerase
Cancer Research
Methyltransferase
Pterostilbene
Cell Survival
Receptor, ErbB-2
Apoptosis
Triple Negative Breast Neoplasms
Resveratrol
Biology
Gene Expression Regulation, Enzymologic
Epigenesis, Genetic
Histones
chemistry.chemical_compound
SIRT1
Sirtuin 1
Cell Line, Tumor
Antineoplastic Combined Chemotherapy Protocols
Stilbenes
Genetics
Humans
Epigenetics
DNA (Cytosine-5-)-Methyltransferases
Regulation of gene expression
Breast cancer cells
Dose-Response Relationship, Drug
DNMTs
Drug Synergism
Cell Cycle Checkpoints
3. Good health
Enzyme Activation
Gene Expression Regulation, Neoplastic
chemistry
Oncology
γ-H2AX
Gene Knockdown Techniques
DNA methylation
Combination
Cancer research
Female
Histone deacetylase
Research Article
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 14712407
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....d0bd956c1d8037d773a747cf87b4b0ff