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Signal Integration by Shadow Enhancers and Enhancer Duplications Varies across the Drosophila Embryo
- Source :
- Cell Reports. 26:2407-2418.e5
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Transcription of developmental genes is controlled by multiple enhancers. Frequently, more than one enhancer can activate transcription from the same promoter in the same cells. How is regulatory information from multiple enhancers combined to determine the overall expression output? We measure nascent transcription driven by a pair of shadow enhancers, each enhancer of the pair separately, and each duplicated, using live imaging in Drosophila embryos. This set of constructs allows us to quantify the input-output function describing signal integration by two enhancers. We show that signal integration performed by these shadow enhancers and duplications varies across the expression pattern, implying that how their activities are combined depends on the transcriptional regulators bound to the enhancers in different parts of the embryo. Characterizing signal integration by multiple enhancers is a critical step in developing conceptual and computational models of gene expression at the locus level, where multiple enhancers control transcription together.
- Subjects :
- 0301 basic medicine
Embryo, Nonmammalian
Kruppel-Like Transcription Factors
Gene Expression Regulation, Developmental
Locus (genetics)
Embryo
Computational biology
Biology
Article
General Biochemistry, Genetics and Molecular Biology
Developmental genes
03 medical and health sciences
Drosophila melanogaster
Enhancer Elements, Genetic
030104 developmental biology
0302 clinical medicine
Expression pattern
Live cell imaging
Transcription (biology)
Gene expression
Animals
Drosophila Proteins
Promoter Regions, Genetic
Enhancer
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....d0a7ae2bcafea3902d5774cfac9b3292