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Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice
- Source :
- Nutrients, Volume 12, Issue 3, Nutrients, Vol 12, Iss 3, p 726 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Obesity is associated with metabolic disorders. Thus, obesity prevention and treatment are essential for health. Antrodia cinnamomea (AC) is a multifunctional medicinal fungus used for the treatment of various diseases and for preventing diet-induced obesity. Leptin deficiency causes over-eating and spontaneous obesity. The concomitant metabolic symptoms are more severe than diet-induced obesity. Here, we used leptin-deficient (ob/ob) mice as an animal model for over-feeding to study the effect of AC on obesity. We fed C57BL/6 mice (WT, ob+/+) and ob/ob mice with AC for four weeks before performing qRT-PCR and immunoblot analysis to elaborate AC-modulated mechanisms. Further, we used Caco-2 cells as a human intestinal epithelial barrier model to examine the effect of AC on intestinal permeability. Our results suggested that AC reduces lipid deposits of the liver and epididymal white adipose tissue (EWAT) by promoting lipid metabolism and inhibiting lipogenesis-associated genes and proteins in ob/ob mice. Moreover, AC effectively repaired intestinal-barrier injury caused by leptin deficiency and enhanced intestinal barrier integrity in Caco-2 cells. Interestingly, AC significantly reduced body weight and EWAT with no compromise on food intake in ob/ob mice. Thus, AC effectively reduced obesity caused by leptin-deficiency and can potentially be used as a nutraceutical for treating obesity.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Antrodia cinnamomea
lcsh:TX341-641
White adipose tissue
Hyperphagia
Article
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
Barrier integrity
Nutrition and Dietetics
Intestinal permeability
Leptin Deficiency
business.industry
Leptin
Lipid metabolism
medicine.disease
Obesity
030104 developmental biology
Endocrinology
Leptin-deficient (ob/ob) mice
030220 oncology & carcinogenesis
Intestinal Barrier
Anti-obesity
business
lcsh:Nutrition. Foods and food supply
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....d09e10d9fd01416a936c3649dad0a13c
- Full Text :
- https://doi.org/10.3390/nu12030726