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Progesterone 5 alpha-reductase in mouse brain
- Source :
- Brain research. 305(2)
- Publication Year :
- 1984
-
Abstract
- We have examined the distribution of progesterone 5 alpha-reductase activity in minces of discrete brain areas from two strains of adult ovariectomized mice known to be differentially responsive to the behavioral effects of exogenously administered 5 alpha-pregnane-3,20-dione (5 alpha-DHP). Minces of freshly dissected midbrain tegmentum (TEG), hypothalamus-preoptic area (HPOA), hippocampus (HIP), and parietal cortex (CTX) were incubated with [4-14C]progesterone in a Krebs-Henseleit bicarbonate buffer solution containing 10 mM glucose for 2 h at 37 degrees C. The [14C]5 alpha-DHP produced was identified by comparison with known standards in paper and thin-layer chromatography systems, derivative formation, and recrystallization to a constant 14C/3H ratio. We found a 6-fold difference in the extent to which various brain areas formed 5 alpha-DHP. The amount of conversion was similar for both strains of mice. When expressed as picomoles of 5 alpha-DHP formed per 2 h per 20 mg of tissue, the following order of activity was observed: TEG greater than HPOA greater than HIP greater than CTX.
- Subjects :
- endocrine system
medicine.medical_specialty
Recrystallization (geology)
3-Hydroxysteroid Dehydrogenases
Ratón
Tegmentum Mesencephali
Bicarbonate
Central nervous system
Mice, Inbred Strains
Biology
Hippocampus
chemistry.chemical_compound
Mice
Species Specificity
Internal medicine
medicine
Hippocampus (mythology)
Animals
Molecular Biology
Progesterone
Cerebral Cortex
Progesterone Reductase
General Neuroscience
5α-Dihydroprogesterone
Brain
Preoptic Area
Endocrinology
medicine.anatomical_structure
chemistry
Ovariectomized rat
Female
Neurology (clinical)
Midbrain tegmentum
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 305
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Brain research
- Accession number :
- edsair.doi.dedup.....d08678e9ebcf22f68545a560a3a74fbd