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Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes
- Source :
- Micromachines, Vol 10, Iss 9, p 575 (2019), Micromachines, Volume 10, Issue 9
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (Rct) value for the different bacteria concentrations under investigation. As a proof-of-concept, Streptococcus mutans (S. mutans) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 103 CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.
- Subjects :
- Materials science
lcsh:Mechanical engineering and machinery
02 engineering and technology
Buffer (optical fiber)
03 medical and health sciences
Printed circuit board
0302 clinical medicine
lab-on-PCB (printed-circuit-board)
lcsh:TJ1-1570
Electrical and Electronic Engineering
Detection limit
immunosensor
Reproducibility
biology
business.industry
Mechanical Engineering
030206 dentistry
direct bacteria detection
Streptococcus mutans (S. mutans)
021001 nanoscience & nanotechnology
biology.organism_classification
Dielectric spectroscopy
electrochemical impedance spectroscopy
Control and Systems Engineering
Electrode
Optoelectronics
0210 nano-technology
business
Biosensor
Bacteria
Subjects
Details
- Language :
- English
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Micromachines
- Accession number :
- edsair.doi.dedup.....d0658824906c1473e13b61c3323bf4b6