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Functional interaction of the human cytomegalovirus IE2 protein with histone deacetylase 2 in infected human fibroblasts
- Source :
- Journal of General Virology. 88:3214-3223
- Publication Year :
- 2007
- Publisher :
- Microbiology Society, 2007.
-
Abstract
- In human cytomegalovirus (HCMV)-infected cells, the 86 kDa immediate-early (IE) 2 protein plays a key role in transactivating downstream viral genes. Recently, IE2 has been shown to interact with histone deacetylase 1 (HDAC1) and HDAC3. HDAC1 recruited by IE2 was required for IE2-mediated autorepression of the major IE (MIE) promoter, whereas IE2–HDAC3 interaction was suggested to relieve the repressive effect of HDAC3 on viral early promoters. However, whether IE2 indeed inhibits HDAC's deacetylation activity on viral promoters and interacts with other HDACs remains unclear. Here, we provide evidence that IE2 functionally interacts with HDAC2 and negates its repressive effect on the viral polymerase promoter. IE2 interacted with HDAC2 in both virus-infected cells and in vitro, and required the conserved C-terminal half for HDAC2 binding. The subcellular localization of HDAC2 was changed in virus-infected cells, showing colocalization with IE2 in viral transcription and replication sites. The overall HDAC2 protein levels and its deacetylation activity slightly increased during the late stages of infection and the IE2-associated deacetylation activity was still sensitive to an HDAC inhibitor, trichostatin A. In transfection assays, however, histone acetylation of the viral polymerase promoter was suppressed by HDAC2, and this was relieved by IE2 binding. Therefore, our data demonstrate that IE2 functionally interacts with HDAC2 and modulates its deacetylation activity on the viral polymerase promoter. Our results also support the idea that interactions of IE2 with several HDACs to modulate the host epigenetic regulation on viral MIE and early promoters are important events in the process of productive infection.
- Subjects :
- viruses
Cytomegalovirus
Down-Regulation
Histone Deacetylase 2
DNA-Directed DNA Polymerase
Hydroxamic Acids
Virus Replication
Histone Deacetylases
Immediate-Early Proteins
Viral Proteins
Transcription (biology)
Virology
Humans
Promoter Regions, Genetic
biology
Histone deacetylase 2
Acetylation
Promoter
Fibroblasts
HDAC3
Molecular biology
HDAC1
Repressor Proteins
Histone
Viral replication
Cytomegalovirus Infections
Trans-Activators
biology.protein
Subjects
Details
- ISSN :
- 14652099 and 00221317
- Volume :
- 88
- Database :
- OpenAIRE
- Journal :
- Journal of General Virology
- Accession number :
- edsair.doi.dedup.....d062be7a074116d65298b7e06cf6a429
- Full Text :
- https://doi.org/10.1099/vir.0.83171-0