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Determination of dimethylarginine levels in rats using HILIC-MS/MS: An in vivo microdialysis study
- Publication Year :
- 2008
- Publisher :
- WILEY-V C H VERLAG GMBH, 2008.
-
Abstract
- Nitric oxide (NO) is one of the most important mediators and neurotransmitters and its levels change under pathological conditions. NO production may be regulated by endogenous nitric oxide synthase (NOS) inhibitors, in particular asymmetric dimethylarginine (ADMA). Most of the interest is focused on ADMA, since this compound is present in plasma and urine and accumulation of ADMA has been described in many disease states but little is known about cerebrospinal fluid (CSF) concentrations of this compound and of its structural isomer symmetric dimethyl-arginine (SDMA). To determine the levels of methylarginines, we here present a new hydrophilic interaction chromatography (HILIC)-MS/MS method for the precise determination of these substances in CSF from microdialysis samples of rat prefrontal cortex (PFC). The method requires only minimal sample preparation and features isotope-labelled internal standards. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
- Subjects :
- Microdialysis
Prefrontal Cortex
Filtration and Separation
Arginine
Tandem mass spectrometry
Analytical Chemistry
Nitric oxide
chemistry.chemical_compound
Cerebrospinal fluid
Tandem Mass Spectrometry
In vivo
SDMA
Animals
Enzyme Inhibitors
Rats, Wistar
Chromatography
Hydrophilic interaction chromatography
MS
Reference Standards
Rats
ADMA
chemistry
Microdialysi
HILIC-MS
Nitric Oxide Synthase
Asymmetric dimethylarginine
Quantitative analysis (chemistry)
Chromatography, Liquid
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d057e2159ea4f227fbfe885dbac6cf98