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Ethanolic extract of roots from Arctium lappa L. accelerates the healing of acetic acid-induced gastric ulcer in rats: Involvement of the antioxidant system
- Source :
- Food and Chemical Toxicology. 51:179-187
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- We evaluate the curative efficacy of the ethanolic extract (EET) of roots from Arctium lappa (bardana) in healing of chronic gastric ulcers induced by 80% acetic acid in rats and additionally studies the possible mechanisms underlying this action. Oral administration of EET (1, 3, 10 and 30mg/kg) reduced the gastric lesion area in 29.2%, 41.4%, 59.3% and 38.5%, respectively, and at 10mg/kg promoted significant regeneration of the gastric mucosa, which was confirmed by proliferating cell nuclear antigen immunohistochemistry. EET (10mg/kg) treatment did not increase the gastric mucus content but restored the superoxide dismutase activity, prevented the reduction of glutathione levels, reduced lipid hydroperoxides levels, inhibited the myeloperoxidase activity and reduced the microvascular permeability. In addition, EET reduced the free radical generation and increased scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals in vitro. Furthermore, intraduodenal EET (10 and 30mg/kg) decreased volume and acidity of gastric secretion. Total phenolic compounds were high in EET (Folin-Ciocalteau assay) and the analysis by liquid chromatography-mass spectrometry revealed that the main compounds present in EET were a serie of hydroxycinnamoylquinic acid isomers. In conclusion, these data reveal that EET promotes regeneration of damaged gastric mucosa, probably through its antisecretory and antioxidative mechanisms.
- Subjects :
- Antioxidant
Free Radicals
DPPH
medicine.medical_treatment
Vascular permeability
Pharmacology
Toxicology
Plant Roots
Antioxidants
Capillary Permeability
Gastric Acid
chemistry.chemical_compound
Acetic acid
Phenols
Oral administration
medicine
Gastric mucosa
Animals
Stomach Ulcer
Rats, Wistar
Acetic Acid
Peroxidase
Ethanol
Plant Extracts
Superoxide Dismutase
General Medicine
Glutathione
Anti-Ulcer Agents
Arctium
Rats
medicine.anatomical_structure
Biochemistry
chemistry
Gastric Mucosa
Arctium lappa
cardiovascular system
Female
lipids (amino acids, peptides, and proteins)
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....d05552eb07088503e18b3cfe21608a2e