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The Role of Slingshot-1L (SSH1L) in the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Cardiomyocyte-Like Cells
- Source :
- Molecules, Molecules, Vol 17, Iss 12, Pp 14975-14994 (2012), Molecules; Volume 17; Issue 12; Pages: 14975-14994
- Publication Year :
- 2012
- Publisher :
- MDPI, 2012.
-
Abstract
- Adult cardiomyocytes (CMs) have very limited capacity to regenerate. Therefore, there is a great interest in developing strategies to treat infarcted CMs that are able to regenerate cardiac tissue and promote revascularization of infarcted zones in the heart. Recently, stem cell transplantation has been proposed to replace infarcted CMs and to restore the function of the affected tissue. This area of research has become very active in recent years due to the huge clinical need to improve the efficacy of currently available therapies. Slingshot (SSH) is a family of protein phosphatases, which can specifically dephosphorylate and reactivate cofilin and inhibit the polymerization of actin filaments and actively involved in cytoskeleton rearrangement. In this study, we found that SSH1L promoted morphology changes of microfilaments during differentiation but was inhibited by the inhibitors of actin polymerization such as cytochalasin D. Overexpression of SSH1L could promote cardiac-specific protein and genes expression. 5-Aza can induce the differentiation of hMSCs into cardiomyocyte-like cells in vitro. We also observed that SSH1L efficiently promotes hMSCs differentiation into cardiomyocyte-like cells through regulation and rearrangement of cytoskeleton. Our work provides evidence that supports the positive role of SSH1L in the mechanism of stem cell differentiation into cardiomyocyte-like cells.
- Subjects :
- Adult
Cytochalasin D
Cellular differentiation
Pharmaceutical Science
Clinical uses of mesenchymal stem cells
Bone Marrow Cells
macromolecular substances
Biology
Article
Analytical Chemistry
mesenchymal stem cells
cardiomyocyte-like cells
5-azacytidine
Slingshot-1L
lcsh:QD241-441
chemistry.chemical_compound
lcsh:Organic chemistry
Drug Discovery
Phosphoprotein Phosphatases
Humans
Cytochalasin
Myocytes, Cardiac
Physical and Theoretical Chemistry
Phosphorylation
Cytoskeleton
Cells, Cultured
Organic Chemistry
Mesenchymal stem cell
Cell Differentiation
Cofilin
Molecular biology
Cell biology
Transplantation
Actin Cytoskeleton
chemistry
Actin Depolymerizing Factors
Gene Expression Regulation
Chemistry (miscellaneous)
Azacitidine
Molecular Medicine
Stem cell
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 17
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....d05035b464ac212bef4c670d8b5680f4