Back to Search
Start Over
Neuronal nicotinic acetylcholine receptor in the hypothalamus: morphological diversity and neuroendocrine regulations
- Source :
- Molecular Brain Research. 49:45-54
- Publication Year :
- 1997
- Publisher :
- Elsevier BV, 1997.
-
Abstract
- The subcellular localization and functional significance of neuronal nicotinic acetylcholine receptor alpha4-subunits were investigated in the rat hypothalamic supraoptic nucleus. A high level of alpha4 mRNA expression was found in the magnocellular neurons in the supraoptic nucleus. Strong immunoreactitivy for alpha4 in neurons of the supraoptic nucleus was detected in the rough endoplasmic reticulum and cytoplasmic matrix, although it was very weak in the Golgi apparatus, except for the transport vesicles. Immunoreactivity for alpha4 was detected in both the pre-synaptic axon terminals and post-synaptic axon terminals. A high level of signals for vasopressin mRNA was detected in the supraoptic nucleus after the animals were injected s.c. with nicotine. These findings suggest that alpha4-containing subtypes are synthesized in the rough endoplasmic reticulum and transported to the plasma membrane and serve as pre- and post-synaptic nicotinic acetylcholine receptors. Nicotine may up-regulate vasopressin gene expression in the supraoptic nucleus, acting through nicotinic acetylcholine receptors.
- Subjects :
- Male
Nicotine
medicine.medical_specialty
Transcription, Genetic
Vasopressins
Hypothalamus
Receptors, Nicotinic
Biology
Polymerase Chain Reaction
Supraoptic nucleus
Cellular and Molecular Neuroscience
Ganglion type nicotinic receptor
Internal medicine
medicine
Animals
RNA, Messenger
Rats, Wistar
Microscopy, Immunoelectron
Molecular Biology
In Situ Hybridization
DNA Primers
Acetylcholine receptor
Neurons
Cell Membrane
Genetic Variation
Immunohistochemistry
Axons
Rats
Up-Regulation
Nicotinic acetylcholine receptor
Endocrinology
Nicotinic agonist
Synapses
Magnocellular cell
Endoplasmic Reticulum, Rough
Alpha-4 beta-2 nicotinic receptor
Supraoptic Nucleus
Subjects
Details
- ISSN :
- 0169328X
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Molecular Brain Research
- Accession number :
- edsair.doi.dedup.....d03536e0592bff338075d156c13ac65d