Back to Search
Start Over
PLP inhibits the activity of interphase centrosomes to ensure their proper segregation in stem cells
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2013
- Publisher :
- The Rockefeller University Press, 2013.
-
Abstract
- PLP levels are regulated by Centrobin and act to suppress mother centrosome maturation by blocking the localization of Polo kinase.<br />Centrosomes determine the mitotic axis of asymmetrically dividing stem cells. Several studies have shown that the centrosomes of the Drosophila melanogaster central brain neural stem cells are themselves asymmetric, organizing varying levels of pericentriolar material and microtubules. This asymmetry produces one active and one inactive centrosome during interphase. We identify pericentrin-like protein (PLP) as a negative regulator of centrosome maturation and activity. We show that PLP is enriched on the inactive interphase centrosome, where it blocks recruitment of the master regulator of centrosome maturation, Polo kinase. Furthermore, we find that ectopic Centrobin expression influenced PLP levels on the basal centrosome, suggesting it may normally function to regulate PLP. Finally, we conclude that, although asymmetric centrosome maturation is not required for asymmetric cell division, it is required for proper centrosome segregation to the two daughter cells.
- Subjects :
- Fluorescent Antibody Technique
Mitosis
Centrosome cycle
chemical and pharmacologic phenomena
Spindle Apparatus
Biology
Protein Serine-Threonine Kinases
Animals, Genetically Modified
03 medical and health sciences
Microtubule
immune system diseases
Report
Chromosome Segregation
Asymmetric cell division
Image Processing, Computer-Assisted
Animals
Drosophila Proteins
Interphase
Research Articles
030304 developmental biology
Pericentriolar material
Centrosome
0303 health sciences
Stem Cells
030302 biochemistry & molecular biology
Polo kinase
Brain
Cell Biology
Cell biology
Spindle apparatus
enzymes and coenzymes (carbohydrates)
Drosophila melanogaster
lipids (amino acids, peptides, and proteins)
Calmodulin-Binding Proteins
biological phenomena, cell phenomena, and immunity
Subjects
Details
- Language :
- English
- ISSN :
- 15408140 and 00219525
- Volume :
- 202
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....d02ddde799ae44ea1ab625b400456046