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RElative QUantitation Inferred by Evaluating Mixtures (REQUIEM)
- Publication Year :
- 2017
-
Abstract
- Motivated by the lack of easily implementable and generally applicable strategies to increase and assess data accuracy, we devised a novel label-free approach, termed REQUIEM, to address challenges in relative quantitation. For comparing the relative amounts of analytes in two samples, a mixture is prepared from aliquots of the samples, and the samples and the mixture are analyzed in parallel according to the intended workflow. Processing of the resulting data using the REQUIEM algorithm yields unbiased analyte fold-changes and associated statistics, allowing several types of errors to be diagnosed or eliminated. Extensive simulations and analysis of carefully prepared standard samples demonstrated the rigorous foundations of REQUIEM. We applied REQUIEM to several real-world analytical techniques and workflows, notably to tandem mass spectrometry analysis by using isomeric oligosaccharides as test analytes. We conclude that REQUIEM can reveal inaccuracies in the data that are difficult to identify by using traditional approaches.
- Subjects :
- 0301 basic medicine
Analyte
Standard sample
Mass spectrometry
Chemistry
Analytical chemistry
Relative quantitation
Precision
Biochemistry
Article
Analytical Chemistry
03 medical and health sciences
030104 developmental biology
Data accuracy
Environmental Chemistry
RNA-Seq
Biological system
Other Chemical Sciences
Spectroscopy
Accuracy
Cancer
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....d02c97fadbb6cb240a5e1f6d1ae0799f