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Sodium Alginate Modified Platinum Nanozymes With Highly Efficient and Robust Oxidase-Like Activity for Antioxidant Capacity and Analysis of Proanthocyanidins
- Source :
- Frontiers in Chemistry, Frontiers in Chemistry, Vol 8 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media SA, 2020.
-
Abstract
- Platinum nanozymes exhibiting highly efficient and robust oxidase-like activity are successfully synthesized and modified using sodium alginate (SA-PtNPs). According to a steady-state dynamic assay, Michaelis-Menton constant (K m ) is calculated as 11.6 μM, indicating that the affinity of SA-PtNPs toward the substrate, 3, 3', 5, 5'-tetramethylbenzidine (TMB), is high. It shows in the paper that SA-PtNPs exhibit a significant oxidant effect on substrate-O2 to produce O 2 • - as an oxidase mimic. Moreover, the oxidase-like activity fluctuated slightly under changes in environmental pH and incubation time, implying that SA can increase the dispersibility and stability of PtNPs. A colorimetric assay for oligomeric proanthocyanidins (OPC) was realized given how few explorations of the former there are. We found that the significant inhibitory effect of OPC on the oxidase-like activity is due to the competitive effect between OPC and TMB for binding to the active site of SA-PtNPs, resulting in a color change. Under optimal conditions, the logarithmic value of the chromogenic difference (ΔA450nm) to OPC concentration was linear (4-32.5 μM, r = 0.999) with a limit of detection (LOD) of 2.0 μM. The antioxidant capacity of OPC obtained by the Soxhlet extraction method from grape seeds was 2.85 U/mg. The recovery from the experiment in which OPC was added to grape seeds ranged from 97.0 to 98.6% (RSDs of 0.5-3.4%), suggesting a high accuracy in OPC detection. These findings are important because OPC is an internationally recognized antioxidant that eliminates free radicals in the human body and, therefore, may prevent a variety of diseases. Thus, we envisage that this Pt nanozyme-based assay may be prevalent for antioxidant capacity evaluation and analytical applications.
- Subjects :
- Antioxidant
oxidase-like activity
medicine.medical_treatment
chemistry.chemical_element
antioxidant capacity
02 engineering and technology
010402 general chemistry
01 natural sciences
colorimetric
sodium alginate
lcsh:Chemistry
medicine
Original Research
Detection limit
Oxidase test
Chromatography
biology
Chromogenic
Chemistry
Active site
Substrate (chemistry)
General Chemistry
021001 nanoscience & nanotechnology
0104 chemical sciences
lcsh:QD1-999
Proanthocyanidin
platinum nanozyme
biology.protein
0210 nano-technology
Platinum
proanthocyanidins
Subjects
Details
- Language :
- English
- ISSN :
- 22962646
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in Chemistry
- Accession number :
- edsair.doi.dedup.....d00cc3f496e9627f0c1445caf27726a7
- Full Text :
- https://doi.org/10.3389/fchem.2020.00654