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LncRNA SOX2-OT regulates proliferation and metastasis of nasopharyngeal carcinoma cells through miR-146b-5p/HNRNPA2B1 pathway
- Source :
- Journal of cellular biochemistry. 120(10)
- Publication Year :
- 2019
-
Abstract
- Nasopharyngeal carcinoma (NPC) is an aggressive malignancy with a high mortality on account of its frequent metastasis and poor prognosis. An extensive body of investigations has proven that long noncoding RNAs are implicated in a variety of biological processes. Although SOX2-OT has been reported to play an oncogenic role in osteosarcoma, the mechanism of SOX2-OT-driven NPC progression is still obscure. The aim of this study was to elucidate the biological function of SOX2-OT and the related possible mechanism in NPC. In our study, SOX2-OT was notably elevated in NPC samples and cells. Further, a high expression level of SOX2-OT was correlated with poor clinical outcomes of NPC. Results from loss-of-function experiments suggested that knockdown of SOX2-OT repressed cell proliferation, arrested cell cycle, facilitated cell apoptosis, and inhibited cell metastasis of NPC. To further investigate the molecular mechanism of SOX2-OT, miR-146b-5p was found to directly bind to SOX2-OT, which mediated the role of SOX2-OT in NPC tumorigenesis. In addition, HNRNPA2B1 was a target of miR-146b-5p and SOX2-OT modulated the expression of HNRNPA2B1 through competitively binding to miR-146b-5p. At last, we discovered that SOX2-OT regulated NPC progression by targeting miR-146b-5p/HNRNPA2B1 pathway, which may provide more innovative targets for the treatment of patients with NPC.
- Subjects :
- 0301 basic medicine
Cell
Mice, Nude
Biology
medicine.disease_cause
Biochemistry
Metastasis
03 medical and health sciences
Mice
0302 clinical medicine
stomatognathic system
SOX2
Cell Line, Tumor
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
otorhinolaryngologic diseases
medicine
Animals
Humans
RNA, Neoplasm
Neoplasm Metastasis
Molecular Biology
Cell Proliferation
Mice, Inbred BALB C
Nasopharyngeal Carcinoma
Cell growth
Nasopharyngeal Neoplasms
Cell Biology
Cell cycle
medicine.disease
Long non-coding RNA
Neoplasm Proteins
stomatognathic diseases
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Nasopharyngeal carcinoma
030220 oncology & carcinogenesis
embryonic structures
Cancer research
RNA, Long Noncoding
sense organs
Carcinogenesis
Subjects
Details
- ISSN :
- 10974644
- Volume :
- 120
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of cellular biochemistry
- Accession number :
- edsair.doi.dedup.....d008a0c93ff13125d471d53e345b05ec