Back to Search
Start Over
Increase of reactive oxygen species generation in cerebral cortex slices after the transiently enhanced metabolic activity
- Source :
- Neuroscience Research. 123:55-64
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Under certain conditions such as hypoxia-reoxygenation, the generation of reactive oxygen species (ROS) increases following hypoxia caused by a decreased oxygen supply. As another hypoxic condition, an excess neural activity status including epileptic seizure induces a decrease in tissue oxygen partial pressure (pO2) caused by enhanced oxygen utilization; however, whether ROS generation increases following the hypoxic status induced by transiently enhanced energy metabolism in brain tissue currently remains unknown. We herein investigated ROS-dependent chemiluminescence in cerebral cortex slices during the restoration of transiently enhanced energy metabolism induced by a high-potassium treatment with tissue pO2 changes and redox balance. ROS generation in the tissue was enhanced after high-potassium-induced hypoxia, but not by the reversed order of the treatment: control-potassium then high-potassium treatment, high-potassium treatment alone, and control-potassium treatment alone. The high-potassium treatment induced a transient decrease in tissue pO2 and a shift in the tissue redox balance towards reduction. The transient shift in the tissue redox balance towards reduction with enhanced metabolic activity and its recovery may correlate with ROS generation. This phenomenon may mimic ROS generation following the hypoxic status induced by transiently enhanced energy metabolism.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Time Factors
Partial Pressure
Apparent oxygen utilisation
In Vitro Techniques
Redox
03 medical and health sciences
Oxygen Consumption
0302 clinical medicine
Slice preparation
Fluorodeoxyglucose F18
Internal medicine
medicine
Animals
Rats, Wistar
Cerebral Cortex
chemistry.chemical_classification
Reactive oxygen species
Luminescent Agents
Dose-Response Relationship, Drug
Chemistry
General Neuroscience
General Medicine
Hypoxia (medical)
Rats
Glucose
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Cerebral cortex
Potassium
Biophysics
Acridines
Autoradiography
Epileptic seizure
medicine.symptom
Energy Metabolism
Reactive Oxygen Species
Metabolic activity
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 01680102
- Volume :
- 123
- Database :
- OpenAIRE
- Journal :
- Neuroscience Research
- Accession number :
- edsair.doi.dedup.....d0078a08e23ef10385dc41c0ec69890d