Back to Search
Start Over
Effect of aging on endothelium-dependent vascular relaxation of isolated human basilar artery to thrombin and bradykinin
- Source :
- Stroke. 21:1039-1043
- Publication Year :
- 1990
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1990.
-
Abstract
- Using strips of human basilar arteries mounted in organ chambers to record isometric tension, we investigated vascular reactivity to thrombin and bradykinin. Both agents produced endothelium-dependent relaxation of basilar artery strips precontracted with phenylephrine but had no effect on resting tension in strips with or without endothelium. The relaxations caused by thrombin were abolished by antithrombin III/heparin, hirudin, and MD805. Thrombin but not bradykinin caused complete tachyphylaxis toward a second exposure. Indomethacin did not inhibit the relaxations induced by thrombin or bradykinin, whereas bromophenacyl bromide and methylene blue did. Aging decreased the relaxation induced by thrombin but did not affect the concentration needed to reach 50% maximal relaxation, nor did it affect the maximal relaxation in response to bradykinin, calcium ionophore A23187, and sodium nitroprusside. Our results suggest that thrombin and bradykinin produce endothelium-dependent relaxations mediated by an endothelium-derived relaxing substance and that the relaxation caused by thrombin is mediated by a proteolytic action on endothelial cells. The decrease in relaxations in response to thrombin with increasing age might be due to a decrease in the number or sensitivity of thrombin receptors on endothelial cells.
- Subjects :
- Adult
Male
Nitroprusside
Aging
medicine.medical_specialty
Adolescent
Endothelium
Indomethacin
Hirudin
Bradykinin
In Vitro Techniques
Tachyphylaxis
Muscle Development
Muscle, Smooth, Vascular
chemistry.chemical_compound
Thrombin
Isometric Contraction
medicine.artery
Internal medicine
medicine
Basilar artery
Humans
Phenylephrine
Calcimycin
Aged
Advanced and Specialized Nursing
business.industry
Middle Aged
Vasodilation
medicine.anatomical_structure
Endocrinology
chemistry
Basilar Artery
Anesthesia
Female
Endothelium, Vascular
Neurology (clinical)
Sodium nitroprusside
Cardiology and Cardiovascular Medicine
business
circulatory and respiratory physiology
medicine.drug
Subjects
Details
- ISSN :
- 15244628 and 00392499
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Stroke
- Accession number :
- edsair.doi.dedup.....cfea21241547a262d0ec46308b620f9e