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Chondroitinase ABC Enhances Integration of Self-Assembled Articular Cartilage, but Its Dosage Needs to Be Moderated Based on Neocartilage Maturity
- Source :
- Cartilage, Cartilage, vol 13, iss 2_suppl
- Publication Year :
- 2020
- Publisher :
- SAGE Publications, 2020.
-
Abstract
- Objective To enhance the in vitro integration of self-assembled articular cartilage to native articular cartilage using chondroitinase ABC. Design To examine the hypothesis that chondroitinase ABC (C-ABC) integration treatment (C-ABCint) would enhance integration of neocartilage of different maturity levels, this study was conducted in 2 phases. In phase I, the impact on integration of 2 treatments, TCL (TGF-β1, C-ABC, and lysyl oxidase like 2) and C-ABCint, was examined via a 2-factor, full factorial design. In phase II, construct maturity (2 levels) and C-ABCint concentration (3 levels) were the factors in a full factorial design to determine whether the effective C-ABCint dose was dependent on neocartilage maturity level. Neocartilages formed or treated per the factors above were placed into native cartilage rings, cultured for 2 weeks, and, then, integration was studied histologically and mechanically. Prior to integration, in phase II, a set of treated constructs were also assayed to provide a baseline of properties. Results In phase I, C-ABCint and TCL treatments synergistically enhanced interface Young’s modulus by 6.2-fold ( P = 0.004) and increased interface tensile strength by 3.8-fold ( P = 0.02) compared with control. In phase II, the interaction of the factors C-ABCint and construct maturity was significant ( P = 0.0004), indicating that the effective C-ABCint dose to improve interface Young’s modulus is dependent on construct maturity. Construct mechanical properties were preserved regardless of C-ABCint dose. Conclusions Applying C-ABCint to neocartilage is an effective integration strategy with translational potential, provided its dose is calibrated appropriately based on implant maturity, that also preserves implant biomechanical properties.
- Subjects :
- Cartilage, Articular
media_common.quotation_subject
Medical Biotechnology
Clinical Sciences
0206 medical engineering
Biomedical Engineering
Chondroitinase ABC
cartilage tissue engineering
integration
Bioengineering
Physical Therapy, Sports Therapy and Rehabilitation
Articular cartilage
02 engineering and technology
Chondroitin ABC Lyase
Biology
biomechanics
Cartilage tissue engineering
self-assembled articular cartilage
Self assembled
03 medical and health sciences
Chondrocytes
Tensile Strength
Immunology and Allergy
Clinical Research papers
030304 developmental biology
media_common
chondroitinase ABC
0303 health sciences
Tissue Engineering
020601 biomedical engineering
Maturity (psychological)
Cell biology
Subjects
Details
- ISSN :
- 19476043 and 19476035
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- CARTILAGE
- Accession number :
- edsair.doi.dedup.....cfe5acb281c1a32dfd5c50ea9df8ce81