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Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway
- Source :
- Evidence-Based Complementary and Alternative Medicine, Vol 2012 (2012), Evidence-based Complementary and Alternative Medicine : eCAM
- Publication Year :
- 2012
- Publisher :
- Hindawi Limited, 2012.
-
Abstract
- Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mechanisms underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin zymography analysis findings further indicated that CAPE downregulated matrix metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and the activation of its downstream signaling molecules p38/MAPK and JNK. These data indicate that CAPE could potentially be used as a chemoagent to prevent oral cancer metastasis.
- Subjects :
- MAPK/ERK pathway
biology
Article Subject
business.industry
p38 mitogen-activated protein kinases
education
Cell migration
lcsh:Other systems of medicine
Matrix metalloproteinase
lcsh:RZ201-999
Focal adhesion
chemistry.chemical_compound
Complementary and alternative medicine
Biochemistry
chemistry
Mitogen-activated protein kinase
Cancer cell
biology.protein
Cancer research
Medicine
business
Caffeic acid phenethyl ester
geographic locations
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17414288
- Volume :
- 2012
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....cfd706bc7c0b4124df26e2edc7551c22