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Caffeic Acid Phenethyl Ester Inhibits Oral Cancer Cell Metastasis by Regulating Matrix Metalloproteinase-2 and the Mitogen-Activated Protein Kinase Pathway

Authors :
Ming-Yung Chou
Chiao-Wen Lin
Yin-Hung Chu
Yueh-Min Lin
Chih-Yu Peng
Yu-Chao Chang
Ming-Ju Hsieh
Shun-Fa Yang
Kun-Tu Yeh
Hui-Wen Yang
Source :
Evidence-Based Complementary and Alternative Medicine, Vol 2012 (2012), Evidence-based Complementary and Alternative Medicine : eCAM
Publication Year :
2012
Publisher :
Hindawi Limited, 2012.

Abstract

Caffeic acid phenethyl ester (CAPE), an active component extracted from honeybee hives, exhibits anti-inflammatory and anticancer activities. However, the molecular mechanism by which CAPE affects oral cancer cell metastasis has yet to be elucidated. In this study, we investigated the potential mechanisms underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin zymography analysis findings further indicated that CAPE downregulated matrix metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and the activation of its downstream signaling molecules p38/MAPK and JNK. These data indicate that CAPE could potentially be used as a chemoagent to prevent oral cancer metastasis.

Details

Language :
English
ISSN :
17414288
Volume :
2012
Database :
OpenAIRE
Journal :
Evidence-Based Complementary and Alternative Medicine
Accession number :
edsair.doi.dedup.....cfd706bc7c0b4124df26e2edc7551c22