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Truncated IGF-1 exerts trophic effects on fetal brain tissue grafts
Truncated IGF-1 exerts trophic effects on fetal brain tissue grafts
- Source :
- Experimental neurology. 108(1)
- Publication Year :
- 1990
-
Abstract
- Truncated IGF-1 (tIGF-1), a form of IGF-1 identified in the human brain, has been suggested, from in vitro experiments, to exert neurotrophic effects on developing fetal brain tissue. We studied the effects of tIGF-1 and IGF-1 on small defined areas of the developing central nervous system by using the in vivo model of intraocular transplantation which allows for direct observations of graft survival and growth. Truncated IGF-1 was found to significantly enhance the growth of fetal spinal cord (Embryonic Day (E) 14) and parietal cortex (E16-17) grafts transplanted to the anterior chamber of the eye of adult rats. tIGF-1 increased the volume of cerebral cortex grafts by approximately 100% and of E14 spinal cord grafts by approximately 50%. E18 spinal cord grafts and hippocampal grafts were not stimulated by tIGF-1 as compared to controls given HSA. Effects in cortex were seen with tIGF-1 using concentrations down to at least 10 ng/μl. Interestingly, intact IGF-1 had no effect on cortical grafts. These findings show for the first time, using an in vivo system, that tIGF-1 is a potent stimulator of growth of grafted fetal cortex cerebri and spinal cord and suggest a possible role for endogenous tIGF-1 in cortical and spinal cord development.
- Subjects :
- Male
medicine.medical_specialty
Transplantation, Heterotopic
Central nervous system
Biology
Hippocampal formation
Eye
Hippocampus
Developmental Neuroscience
Somatomedins
Internal medicine
Cortex (anatomy)
Parietal Lobe
medicine
Animals
Insulin-Like Growth Factor I
Nerve Tissue
Neurons
Rats, Inbred Strains
Human brain
Anatomy
Spinal cord
Rats
Transplantation
medicine.anatomical_structure
Endocrinology
Neurology
Spinal Cord
Cerebral cortex
biology.protein
Neuroglia
Cell Division
Neurotrophin
Subjects
Details
- ISSN :
- 00144886
- Volume :
- 108
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Experimental neurology
- Accession number :
- edsair.doi.dedup.....cfc33557116919274c1613ea7242c5d2