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Universal direct PCR amplification system: a time- and cost-effective tool for high-throughput applications

Authors :
Anis Ben-Amar
Ahmed Mliki
Souheib Oueslati
Source :
3 Biotech. 7
Publication Year :
2017
Publisher :
Springer Science and Business Media LLC, 2017.

Abstract

Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications. The direct PCR technique established here represents an excellent alternative to traditional amplification methods used for real-time detection. Since this approach was efficiently and universally applied for high-throughput molecular screening, its implementation will offer new insights for several investigations in human health, biomedical diagnosis, plant biotechnology, as well as in applied environmental and food microbiology.

Details

ISSN :
21905738 and 2190572X
Volume :
7
Database :
OpenAIRE
Journal :
3 Biotech
Accession number :
edsair.doi.dedup.....cfc29aa85cd546a58d8a18cdf31893b3
Full Text :
https://doi.org/10.1007/s13205-017-0890-7