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Universal direct PCR amplification system: a time- and cost-effective tool for high-throughput applications
- Source :
- 3 Biotech. 7
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Taking into account the limits of current genotyping methodologies, we have established a versatile direct PCR method on intact microtissue samples without prior DNA isolation. A simple and standard protocol was developed and validated on a wide range of living organisms including bacterial and fungal strains, plant species and human samples. This allows reliable amplification of target genomic DNA fragment directly from source material using minimal amount of tissue which makes DNA purification irrelevant for a number of biological applications. The direct PCR technique established here represents an excellent alternative to traditional amplification methods used for real-time detection. Since this approach was efficiently and universally applied for high-throughput molecular screening, its implementation will offer new insights for several investigations in human health, biomedical diagnosis, plant biotechnology, as well as in applied environmental and food microbiology.
- Subjects :
- 0301 basic medicine
Molecular screening
business.industry
030231 tropical medicine
030106 microbiology
Computational biology
Environmental Science (miscellaneous)
Biology
Protocols and Methods
Agricultural and Biological Sciences (miscellaneous)
DNA extraction
System a
law.invention
Biotechnology
03 medical and health sciences
genomic DNA
0302 clinical medicine
law
business
Throughput (business)
Applications of PCR
Genotyping
Polymerase chain reaction
Subjects
Details
- ISSN :
- 21905738 and 2190572X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- 3 Biotech
- Accession number :
- edsair.doi.dedup.....cfc29aa85cd546a58d8a18cdf31893b3
- Full Text :
- https://doi.org/10.1007/s13205-017-0890-7