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AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling
- Source :
- Journal of Experimental Botany 67 (2016) 1, Journal of Experimental Botany, Journal of Experimental Botany, 67(1), 3383-3396
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Highlight The transcription factor WRKY22 increases susceptibility to aphids in Arabidopsis via the suppression of salicylic acid signalling.<br />Aphids induce many transcriptional perturbations in their host plants, but the signalling cascades responsible and the effects on plant resistance are largely unknown. Through a genome-wide association (GWA) mapping study in Arabidopsis thaliana, we identified WRKY22 as a candidate gene associated with feeding behaviour of the green peach aphid, Myzus persicae. The transcription factor WRKY22 is known to be involved in pathogen-triggered immunity, and WRKY22 gene expression has been shown to be induced by aphids. Assessment of aphid population development and feeding behaviour on knockout mutants and overexpression lines showed that WRKY22 increases susceptibility to M. persicae via a mesophyll-located mechanism. mRNA sequencing analysis of aphid-infested wrky22 knockout plants revealed the up-regulation of genes involved in salicylic acid (SA) signalling and down-regulation of genes involved in plant growth and cell-wall loosening. In addition, mechanostimulation of knockout plants by clip cages up-regulated jasmonic acid (JA)-responsive genes, resulting in substantial negative JA–SA crosstalk. Based on this and previous studies, WRKY22 is considered to modulate the interplay between the SA and JA pathways in response to a wide range of biotic and abiotic stimuli. Its induction by aphids and its role in suppressing SA and JA signalling make WRKY22 a potential target for aphids to manipulate host plant defences.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Arabidopsis thaliana
transcription factors
Physiology
Myzus persicae
Arabidopsis
Plant Science
Wiskundige en Statistische Methoden - Biometris
01 natural sciences
chemistry.chemical_compound
touch
Laboratorium voor Plantenfysiologie
Laboratory of Entomology
Aphid
biology
Jasmonic acid
Entomology & Disease Management
food and beverages
PE&RC
Cell biology
Biometris
BIOS Applied Metabolic Systems
Salicylic Acid
Laboratory of Plant Physiology
Signal Transduction
Research Paper
Cyclopentanes
plant–insect interaction
BIOS Applied Bioinformatics
03 medical and health sciences
Botany
Animals
BIOS Plant Development Systems
Herbivory
Oxylipins
Mathematical and Statistical Methods - Biometris
Gene
Transcription factor
Arabidopsis Proteins
biochemical phenomena, metabolism, and nutrition
Laboratorium voor Entomologie
biology.organism_classification
mechanostimulation
030104 developmental biology
MRNA Sequencing
chemistry
Aphids
EPS
plant resistance to aphids
plant-insect interaction
Salicylic acid
Genome-Wide Association Study
Transcription Factors
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....cfab56b4d6fa76de19b8a6b55677546a