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Association of variable number tandem repeats polymorphism in the IL-4 gene with end-stage renal disease in children
- Source :
- Egyptian Journal of Medical Human Genetics; Vol 19, No 3 (2018); 191–195, Egyptian Journal of Medical Human Genetics, Vol 19, Iss 3, Pp 191-195 (2018)
- Publication Year :
- 2018
- Publisher :
- The Egyptian Society of Human Genetics, 2018.
-
Abstract
- Background : End stage renal disease (ESRD) is a common cause of morbidity and mortality among children. Interleukin 4 is a cytokine that might influence the progression of chronic kidney disease (CKD) to end stage renal disease. There are variable number of tandem repeats (VNTRs) in IL4 gene that could play major roles in genetic predisposition to some diseases. Aim of the study: The purpose of this study is to detect the association of allelic variant in intron 3 VNTR-IL4 gene with the end stage renal disease and if these variants could be considered as risk markers for this disease. Subjects and methods : The study was conducted on fifty-five children with CKD and fifty healthy children served as controls. All participants were genotyped for intron 3 VNTR by Polymerase Chain Reaction. Results : The frequency of intron 3 VNTR-IL4 P1P2 + P2P2 genotypes was significantly higher in ESRDchildren than those with P1P1 genotype (88.7% vs. 15.4%, OR 43; 95% CI 13–134, P value < 0.001). Also, the frequency of P2 allele was significantly higher in ESRD-children compared with healthy controls (70.9% vs. 8%, OR 28; 95% CI 12–64, P value < 0.001). Furthermore, a significantly higher frequencies of P1P1 genotype and P1 allele among the control group were demonstrated (84.6% vs. 11.3%, P < 0.001 and 92% vs. 29.1%, P < 0.001, respectively). Conclusion : we concluded that the P2 allele is an allelic variant predisposing to ESRD in children with CKD and it could be considered a risk factor for the development of ESRD. Keywords : VNTR-IL4 ESRD PCR Children Gene polymorphism
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
lcsh:QH426-470
Disease
Biology
Bioinformatics
urologic and male genital diseases
Gastroenterology
End stage renal disease
03 medical and health sciences
0302 clinical medicine
Internal medicine
Genotype
medicine
Genetic predisposition
Allele
Genetics (clinical)
lcsh:R5-920
medicine.disease
Variable number tandem repeat
lcsh:Genetics
030104 developmental biology
030220 oncology & carcinogenesis
VNTR-IL4 ESRD PCR Children Gene polymorphism
Gene polymorphism
lcsh:Medicine (General)
Kidney disease
Subjects
Details
- Language :
- English
- ISSN :
- 11108630
- Database :
- OpenAIRE
- Journal :
- Egyptian Journal of Medical Human Genetics
- Accession number :
- edsair.doi.dedup.....cf951be55c7c018a80ee9c90b4cae546