Back to Search
Start Over
T cell senescence and impaired CMV-specific response are associated with infection risk in kidney transplant recipients
- Source :
- Hum Immunol, Human immunology, vol 83, iss 4
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Older kidney transplant recipients demonstrate increased rates of infection, and lower rates of rejection, compared with younger kidney transplant recipients. However, the mechanism behind this observation remains unknown. To develop a multifaceted view of age-associated immune dysfunction, we determined the function and phenotype of T cells predisposing to vulnerability to infection on a molecular level. Overlapping peptide pools representing the dominant CMV antigens were used to stimulate PBMC collected from 51 kidney transplant recipients, using cytokine secretion to determine specificity and intensity of response. Staphylococcal endotoxin B (SEB) was analyzed in parallel. To define immune cell subsets, we used single cell RNA sequencing (scRNAseq) to evaluate cellular surface markers and gene expression. We found increased frequency of SEB- and CMV-specific T cells was associated with freedom from infection, especially in older patients. Spatialized t-SNE analysis revealed decreased frequency of naïve T cells, increased frequency of TEMRA cells, and decreased frequency of IFNγ secreting T cells in patients with infection. Application of scRNAseq analysis revealed increased frequency of terminally differentiated T cells expressing NK-associated receptors and inhibitory markers. These findings offer unique insight into the mechanism behind vulnerability to infection in the kidney transplant recipient, revealing a specific T cell subtype of impaired antigen response and terminal effector phenotype as markers of T cell senescence.
- Subjects :
- Aging
Kidney Disease
Exhaustion
T-Lymphocytes
Mononuclear
Immunology
Renal and urogenital
Senescence
IFN
Article
Vaccine Related
Clinical Research
Biodefense
Leukocytes
Humans
2.1 Biological and endogenous factors
Immunology and Allergy
Aetiology
Cellular Senescence
Aged
Inflammation
Transplantation
screening and diagnosis
Prevention
Inflammatory and immune system
T cell
Organ Transplantation
General Medicine
Kidney Transplantation
Transplant Recipients
4.1 Discovery and preclinical testing of markers and technologies
Detection
Emerging Infectious Diseases
Infectious Diseases
Cytomegalovirus Infections
Leukocytes, Mononuclear
Biomarkers
IFNγ
Subjects
Details
- ISSN :
- 01988859
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Human Immunology
- Accession number :
- edsair.doi.dedup.....cf748e6bf39e8ea8c8baa3a14ae89eb4
- Full Text :
- https://doi.org/10.1016/j.humimm.2022.01.016