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Development of novel hepatitis B virus capsid inhibitor using in silico screening

Authors :
Michiyo Hayakawa
Akihiro Tamori
Norifumi Kawada
Hideaki Umeyama
Yoshiki Murakami
Toshihito Tanahashi
Yoshihiko Yano
Masaru Enomoto
Mitsuo Iwadate
Source :
Biochemical and biophysical research communications. 463(4)
Publication Year :
2015

Abstract

Antiviral therapy for chronic hepatitis B that uses nucleos(t)ide analogue is considered effective. However, most drugs of this class frequently result in viral relapse after cessation of therapy as well as the emergence of resistance, thereby limiting their clinical use. In order to increase the therapeutic efficiency of chronic hepatitis B treatments, it is important to survey novel (chemical) reagents targeting other stages of the viral replication process. The aim of this study was to identify novel capsid inhibitor candidates using in silico screening. We discovered four such candidates that decreased the levels of HBV DNA and HBsAg in vitro. These four capsid inhibitor candidates did not induce cell toxicity even at high concentrations. Results from docking simulation showed that the candidates bounded with high affinity with the capsid protein hydrophobic binding site. Identifying direct acting HBV core protein inhibitors increases the likelihood that novel medicines can be developed that allows the combination of novel anti-viral drugs and nucleos(t)ide analogue or interferon for HBV treatment.

Details

ISSN :
10902104
Volume :
463
Issue :
4
Database :
OpenAIRE
Journal :
Biochemical and biophysical research communications
Accession number :
edsair.doi.dedup.....cf61d8b032bfba897ab06f1883a8bc7b