Additional file 6 of The ESCRT-III isoforms CHMP2A and CHMP2B display different effects on membranes upon polymerization

Additional file 6: Figure S5. Deformation of bare vesicles and vesicles covered with CHMP proteins. (A) Reduction of vesicle height under increasing force for bare vesicles. ‘Zero’ force increment represents the minimum imaging force (~ 150 pN). (B) Example of deformation for a ~ 60 nm vesicle over increasing force up to 80% of the initial imaging force. (C) Represents the transformation of vesicle height to relative height for each point for the curve in D. (D) represents the relative height vs force increment for all the curves from panel A for bare SUVs. (E) represents the relative height vs force increment for SUV covered with CHMP2B (left), CHMP2B + CHMP3 (middle) and CHMP2A + CHMP3 (right).