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To Explore the Inhibitory Mechanism of Quercetin in Thyroid Papillary Carcinoma through Network Pharmacology and Experiments
- Source :
- Disease Markers. 2022:1-11
- Publication Year :
- 2022
- Publisher :
- Hindawi Limited, 2022.
-
Abstract
- Quercetin, a flavonoid with anti-inflammatory and anticancer properties, is expected to be an innovative anticancer therapeutic agent for papillary thyroid carcinoma (PTC). However, the downstream signaling pathways that mediate quercetin-dependent anticancer properties remain to be deciphered. Herein, potential targets of quercetin were screened with several bioinformatic avenues including PharmMapper, Gene Expression Omnibus (GEO) database, protein–protein interaction (PPI) network, and molecular docking. Besides, western blot, CCK-8 transwell analysis of migration and invasion, flow cytometric analysis, and colony formation assays were performed to investigate the underlying mechanism. We found four core nodes (MMP9, JUN, SPP1, and HMOX1) by constructing a PPI network with 23 common targets. Through functional enrichment analysis, we confirmed that the above four target genes are enriched in the TNF, PI3K-AKT, and NF-κB signaling pathways, which are involved in the inflammatory microenvironment and inhibit the development and progression of tumors. Furthermore, molecular docking results demonstrated that quercetin shows strong binding efficiency with the proteins encoded by these 4 key proteins. Finally, quercetin displayed strong antitumor efficacy in PTC cell lines. In this research, we demonstrated the application of network pharmacology in evaluating the mechanisms of action and molecular targets of quercetin, which regulates a variety of proteins and signaling pathways in PTC. These data might explain the mechanism underlying the anticancer effects of quercetin in PTC.
- Subjects :
- Article Subject
Biochemistry (medical)
Clinical Biochemistry
General Medicine
Network Pharmacology
Carcinoma, Papillary
Molecular Docking Simulation
Phosphatidylinositol 3-Kinases
Thyroid Cancer, Papillary
Tumor Microenvironment
Genetics
Humans
Quercetin
Thyroid Neoplasms
Molecular Biology
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 18758630 and 02780240
- Volume :
- 2022
- Database :
- OpenAIRE
- Journal :
- Disease Markers
- Accession number :
- edsair.doi.dedup.....cf47278021f740ebeaf06b97e1090007