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Hypothalamic transcriptome analysis reveals the neuroendocrine mechanisms in controlling broodiness of Muscovy duck (Cairina moschata)
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 5, p e0207050 (2019)
- Publication Year :
- 2018
-
Abstract
- Broodiness, one of the maternal behaviors and instincts for natural breeding in birds, is an interesting topic in reproductive biology. Broodiness in poultry is characterized by persistent nesting, usually associated with cessation of egg laying. The study of avian broodiness is essential for bird conservation breeding and commercial poultry industry. In this study, we examined the hypothalamus transcriptome of Muscovy duck in three reproductive stages, including egg-laying anaphase (LA), brooding prophase (BP) and brooding metaphase (BM). Differences in gene expression during the transition from egg-laying to broodiness were examined, and 155, 379, 292 differently expressed genes (DEGs) were obtained by pairwise comparisons of LA-vs-BP, LA-vs-BM and BP-vs-BM, respectively (fold changeā„ 1.5, P < 0.05). Gene Ontology Term (GO) enrichment analysis suggested a possible role of oxidative stress in the hypothalamus might invoke reproductive costs that potentially change genes expression. KEGG analysis revealed glutamatergic synapse, dopaminergic synapse, serotonergic synapse and GABAergic synapse pathway were significantly enriched, and regulator genes were identified. Eight gene expression patterns were illustrated by trend analysis and further clustered into three clusters. Additional six hub genes were identified through combining trend analysis and protein-protein interaction (PPI) analysis. Our results suggested that the cyclical mechanisms of reproductive function conversion include effects of oxidative stress, biosynthesis of neurotransmitters or their receptors, and interactions between glucocorticoids and thyroid hormones and regulatory genes. These candidate genes and biological pathways may be used as targets for artificial manipulation and marker-assisted breeding in the reproductive behavior.
- Subjects :
- 0301 basic medicine
Candidate gene
Physiology
Molecular biology
Waterfowl
Gene Expression
Nervous System
Biochemistry
Poultry
Transcriptome
Sexual Behavior, Animal
Sequencing techniques
Mathematical and Statistical Techniques
Protein Interaction Mapping
Medicine and Health Sciences
Gene Regulatory Networks
Protein Interaction Maps
Regulator gene
Regulation of gene expression
Genetics
Principal Component Analysis
Multidisciplinary
biology
Statistics
Eukaryota
Brain
Cairina moschata
RNA sequencing
04 agricultural and veterinary sciences
Genomics
Electrophysiology
Ducks
Dopaminergic synapse
Vertebrates
Physical Sciences
Medicine
Female
Glutamatergic synapse
Anatomy
Transcriptome Analysis
Research Article
Science
Hypothalamus
Zoology
Neurophysiology
Egg laying
Birds
03 medical and health sciences
Reproductive biology
DNA-binding proteins
Animals
Gene Regulation
Statistical Methods
Gene
Metaphase
Gene Expression Profiling
0402 animal and dairy science
Organisms
Computational Biology
Reproducibility of Results
Biology and Life Sciences
Proteins
biology.organism_classification
Genome Analysis
040201 dairy & animal science
Neurosecretory Systems
Broodiness
Regulatory Proteins
Gene expression profiling
Research and analysis methods
030104 developmental biology
Gene Ontology
Molecular biology techniques
Gene Expression Regulation
Fowl
Amniotes
Synapses
Multivariate Analysis
Mathematics
Neuroscience
Transcription Factors
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....cf299c1955b630351ee63827119d5654