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Developing Gold Nanoparticles-Conjugated Aflatoxin B1 Antifungal Strips
- Source :
- Articles, International Journal of Molecular Sciences, Volume 20, Issue 24
- Publication Year :
- 2019
- Publisher :
- Technological University Dublin, 2019.
-
Abstract
- Lateral flow immunochromatographic assays are a powerful diagnostic tool for point-of-care tests, based on their simplicity, specificity, and sensitivity. In this study, a rapid and sensitive gold nanoparticle (AuNP) immunochromatographic strip is produced for detecting aflatoxin B1 (AFB1) in suspicious fungi-contaminated food samples. The 10 nm AuNPs were encompassed by bovine serum albumin (BSA) and AFB1 antibody. Thin-layer chromatography, gel electrophoresis and nuclear magnetic resonance spectroscopy were employed for analysing the chemical complexes. Various concentrations of AFB1 antigen (0&ndash<br />16 ng/mL) were tested with AFB1 antibody&ndash<br />BSA&ndash<br />AuNPs (conjugated AuNPs) and then analysed by scanning electron microscopy, ultraviolet&ndash<br />visible spectroscopy, and Zetasizer. The results showed that the AFB1 antibody was coupled to BSA by the N-hydroxysuccinimide ester method. The AuNPs application has the potential to contribute to AFB1 detection by monitoring a visible colour change from red to purple-blue, with a detection limit of 2 ng/mL in a 96-well plate. The lateral flow immunochromatographic strip tests are rapid, taking less than 10 min., and they have a detection capacity of 10 ng/g. The smartphone analysis of strips provided the results in 3 s, with a detection limit of 0.3 ng/g for AFB1 when the concentration was below 10 ng/g. Excellent agreement was found with AFB1 determination by high-performance liquid chromatography in the determination of AFB1 among 20 samples of peanuts, corn, rice, and bread.
- Subjects :
- Aflatoxin
Antifungal Agents
Proton Magnetic Resonance Spectroscopy
Nanoparticle
Metal Nanoparticles
gold nano particles
Food Contamination
02 engineering and technology
Conjugated system
01 natural sciences
Catalysis
Article
Chromatography, Affinity
Inorganic Chemistry
Physical and Theoretical Chemistry
Bovine serum albumin
Molecular Biology
Biology
Spectroscopy
Chromatography, High Pressure Liquid
Serum Albumin
Reagent Strips
Gel electrophoresis
Detection limit
Chromatography
biology
Molecular Structure
Chemistry
010401 analytical chemistry
Organic Chemistry
General Medicine
Nuclear magnetic resonance spectroscopy
021001 nanoscience & nanotechnology
0104 chemical sciences
Computer Science Applications
Colloidal gold
aflatoxin B1
biology.protein
Gold
0210 nano-technology
lateral flow immunochromatographic strips
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Articles, International Journal of Molecular Sciences, Volume 20, Issue 24
- Accession number :
- edsair.doi.dedup.....cf22f42721eae7f12d0be9152e35af14