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Aminoacylation identity switch of turnip yellow mosaic virus RNA from valine to methionine results in an infectious virus
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 93(22)
- Publication Year :
- 1996
-
Abstract
- The turnip yellow mosaic virus genomic RNA terminates at its 3' end in a tRNA-like structure that is capable of specific valylation. By directed mutation, the aminoacylation specificity has been switched from valine to methionine, a novel specificity for viral tRNA-like structures. The switch to methionine specificity, assayed in vitro under physiological buffer conditions with wheat germ methionyl-tRNA synthetase, required mutation of the anticodon loop and the acceptor stem pseudoknot. The resultant methionylatable genomes are infectious and stable in plants, but genomes that lack strong methionine acceptance (as previously shown with regard to valine acceptance) replicate poorly. The results indicate that amplification of turnip yellow mosaic virus RNA requires aminoacylation, but that neither the natural (valine) specificity nor interaction specifically with valyl-tRNA synthetase is crucial.
- Subjects :
- Methionine—tRNA ligase
viruses
Aminoacylation
Methionine-tRNA Ligase
RNA, Transfer, Amino Acyl
chemistry.chemical_compound
Structure-Activity Relationship
Methionine
Valine
Tymovirus
Genetics
Multidisciplinary
Turnip yellow mosaic virus
biology
Virion
RNA
biology.organism_classification
Kinetics
chemistry
Biochemistry
Transfer RNA
Mutagenesis, Site-Directed
Nucleic Acid Conformation
RNA, Viral
Pseudoknot
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 93
- Issue :
- 22
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....cf22bc85afd65a85bf1f057cb6ca13ff