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Upregulation of heme oxygenase 1 (HO-1) attenuates kidney damage, oxidative stress and inflammatory reaction during renal ischemia/ reperfusion injury
- Source :
- General physiology and biophysics. 37(2)
- Publication Year :
- 2018
-
Abstract
- The current study investigated the effect of upregulation of heme oxygenase 1 (HO-1) by cobalt protoporphyrin (CoPP) on renal dysfunctions in renal ischemia/reperfusion (I/R) injury and its underlying mechanisms. 72 male Sprague Dawley rats were divided into 4 groups: sham group, ischemic group (left 45-min renal ischemia), CoPP-before group (as ischemic group with CoPP 20 mg/kg 30 min before ischemia) and CoPP-after group (as ischemic group with CoPP 20 mg/kg 20 min after ischemia). Serum creatinine, urea and TGF-β1 and markers of redox state (MDA, SOD, GSH and CAT), nitric oxide (NO), TGF-β1 and HO-1 in kidney tissues were measured. Serum creatinine and urea levels were significantly increased in ischemic group and attenuated in CoPP-treated groups (p < 0.05). Also, markers of redox state showed significant deteriorations in ischemic group which were improved significantly in CoPP-treated groups (p < 0.05). HO-1 expression in kidney tissues showed significant increase in ischemic group and showed more significant increase in CoPP-treated groups (p < 0.05). Moreover, serum and renal TGF-β1 levels were significantly increased in ischemic group and attenuated in CoPP-treated groups (p ≶ 0.05). We concluded that up-regulation of HO-1 by CoPP treatment before and after renal I/R injury improved the kidney function and morphology and this might be due to impairment of oxidative stress and inflammatory cytokines in kidney tissues.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Physiology
Biophysics
Ischemia
Renal function
Protoporphyrins
medicine.disease_cause
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Random Allocation
Internal medicine
Medicine
Animals
Inflammation
Creatinine
Kidney
Renal ischemia
business.industry
General Medicine
medicine.disease
COPP
Rats
Up-Regulation
Heme oxygenase
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Reperfusion Injury
Kidney Diseases
business
Oxidative stress
Heme Oxygenase-1
Subjects
Details
- ISSN :
- 02315882
- Volume :
- 37
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- General physiology and biophysics
- Accession number :
- edsair.doi.dedup.....cf0ae3107abbcfeab05351a03b6b8042