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Identification of Streptococcus intermedius Central Nervous System Infection by Use of PCR and Electrospray Ionization Mass Spectrometry
- Publication Year :
- 2012
- Publisher :
- American Society for Microbiology, 2012.
-
Abstract
- We describe the utility of PCR and electrospray ionization with mass spectrometry (PCR/ESI-MS) of culture-negative cerebrospinal fluid (CSF) in order to identify Gram-positive cocci noted on a Gram stain of CSF from a previously healthy 26-year-old man with community-acquired pneumonia (CAP) and multiple brain abscesses. CSF samples were obtained 2 weeks apart, first by lumbar puncture and 2 weeks later from an external ventricular drain that was inserted into the right ventricle. Both CSF cultures were negative. A Gram stain of bronchoalveolar lavage (BAL) fluid was notable for many Gram-positive cocci (GPC), but cultures of BAL fluid and subcarinal lymph node biopsy tissue were negative. PCR/ESI-MS detected Streptococcus intermedius , a common cause of brain abscesses, in both CSF samples as well as in the fixed tissue from the biopsy. This unique case confirms S. intermedius pulmonary infection as the source of metastatic CNS infection and reveals the potential of PCR/ESI-MS to detect a streptococcal pathogen not captured by conventional cultures.
- Subjects :
- Microbiology (medical)
Adult
Male
Pathology
medicine.medical_specialty
Spectrometry, Mass, Electrospray Ionization
Brain Abscess
Case Reports
Streptococcus intermedius
Polymerase Chain Reaction
law.invention
Cerebrospinal fluid
Central Nervous System Infections
law
Streptococcal Infections
Biopsy
medicine
Pneumonia, Bacterial
Humans
Brain abscess
Cerebrospinal Fluid
Bacteriological Techniques
biology
medicine.diagnostic_test
biology.organism_classification
medicine.disease
Subcarinal Lymph Node
Pneumonia
Bronchoalveolar lavage
Gram staining
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....cf0955359ad0217abea84f0ed23c3c12