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Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa
- Source :
- BMC Developmental Biology, Vol 11, Iss 1, p 31 (2011), BMC Developmental Biology
- Publisher :
- Springer Nature
-
Abstract
- Background The metamorphosis of the spionid polychaete Pseudopolydora vexillosa includes spontaneous settlement onto soft-bottom habitats and morphogenesis that can be completed in a very short time. A previous study on the total changes to the proteome during the various developmental stages of P. vexillosa suggested that little or no de novo protein synthesis occurs during metamorphosis. In this study, we used multicolor fluorescence detection of proteins in 2-D gels for differential analysis of proteins and phosphoproteins to reveal the dynamics of post-translational modification proteins in this species. A combination of affinity chromatography, 2D-PAGE, and mass spectrometry was used to identify the phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles. Results We reproducibly detected 210, 492, and 172 phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles, respectively. The highest percentage of phosphorylation was observed during the competent larval stage. About 64 stage-specific phosphoprotein spots were detected in the competent stage, and 32 phosphoproteins were found to be significantly differentially expressed in the three stages. We identified 38 phosphoproteins, 10 of which were differentially expressed during metamorphosis. These phosphoproteins belonged to six categories of biological processes: (1) development, (2) cell differentiation and integrity, (3) transcription and translation, (4) metabolism, (5) protein-protein interaction and proteolysis, and (6) receptors and enzymes. Conclusion This is the first study to report changes in phosphoprotein expression patterns during the metamorphosis of the marine polychaete P. vexillosa. The higher degree of phosphorylation during the process of attaining competence to settle and metamorphose may be due to fast morphological transitions regulated by various mechanisms. Our data are consistent with previous studies showing a high percentage of phosphorylation during competency in the barnacle Balanus amphitrite and the bryozoan Bugula neritina. The identified phosphoproteins may play an important role during metamorphosis, and further studies on the location and functions of important proteins during metamorphosis are warranted.
- Subjects :
- Proteome
media_common.quotation_subject
Zoology
Bugula neritina
Biology
Proteomics
Chromatography, Affinity
Mass Spectrometry
Animals
Electrophoresis, Gel, Two-Dimensional
Juvenile animal
Metamorphosis
lcsh:QH301-705.5
media_common
Larva
Metamorphosis, Biological
Polychaeta
Phosphoproteins
Spionida
biology.organism_classification
Molecular biology
lcsh:Biology (General)
Protein Processing, Post-Translational
Developmental biology
Research Article
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 1471213X
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Developmental Biology
- Accession number :
- edsair.doi.dedup.....cf02bde33071c93bd605fb794c4159f8
- Full Text :
- https://doi.org/10.1186/1471-213x-11-31