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Ultrasensitive detection of HIV-1 p24 antigen using nanofunctionalized surfaces in a capacitive immunosensor

Ultrasensitive detection of HIV-1 p24 antigen using nanofunctionalized surfaces in a capacitive immunosensor

Authors :
Martin Hedström
Indira Hewlett
Kosin Teeparuksapun
Eric Y Wong
Bo Mattiasson
Shixing Tang
Source :
Analytical chemistry. 82(20)
Publication Year :
2010

Abstract

The HIV-1 capsid protein, p24 antigen, is of considerable diagnostic interest because following HIV exposure it is detectable several days earlier than host-generated HIV antibodies (which are the target of almost all current tests used in the field) and can be used to design very sensitive assays without the need for PCR. Here, we present an ultrasensitive capacitive immunosensor that is capable of detecting subattogram per milliliter concentrations of p24 antigen, which to our knowledge is the lowest level of detection ever reported. Dilution studies using p24-spiked human plasma samples indicate that the immunosensor is robust against the interfering effects of a complex biological matrix. Moreover, the capacitive immunosensor assay is rapid (

Details

ISSN :
15206882
Volume :
82
Issue :
20
Database :
OpenAIRE
Journal :
Analytical chemistry
Accession number :
edsair.doi.dedup.....cf02a987493d87fe9b41579a0261ce00