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Ultrasensitive detection of HIV-1 p24 antigen using nanofunctionalized surfaces in a capacitive immunosensor
Ultrasensitive detection of HIV-1 p24 antigen using nanofunctionalized surfaces in a capacitive immunosensor
- Source :
- Analytical chemistry. 82(20)
- Publication Year :
- 2010
-
Abstract
- The HIV-1 capsid protein, p24 antigen, is of considerable diagnostic interest because following HIV exposure it is detectable several days earlier than host-generated HIV antibodies (which are the target of almost all current tests used in the field) and can be used to design very sensitive assays without the need for PCR. Here, we present an ultrasensitive capacitive immunosensor that is capable of detecting subattogram per milliliter concentrations of p24 antigen, which to our knowledge is the lowest level of detection ever reported. Dilution studies using p24-spiked human plasma samples indicate that the immunosensor is robust against the interfering effects of a complex biological matrix. Moreover, the capacitive immunosensor assay is rapid (
- Subjects :
- Immunoassay
Chromatography
biology
medicine.diagnostic_test
Chemistry
Capacitive immunosensor
Cell Membrane
Human immunodeficiency virus (HIV)
HIV Core Protein p24
Metal Nanoparticles
medicine.disease_cause
Microscopy, Atomic Force
Analytical Chemistry
Matrix (chemical analysis)
Hiv 1 p24
Antigen
Microscopy, Electron, Transmission
Flow Injection Analysis
biology.protein
medicine
HIV-1
Gold
Antibody
Biosensor
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 82
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....cf02a987493d87fe9b41579a0261ce00