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Methylation of the hsa-miR-124, SOX1, TERT, and LMX1A genes as biomarkers for precursor lesions in cervical cancer
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia.<br />Objectives The methylation profile of genes in precursor lesions in cervical cancer was characterized to improve screening techniques for high-grade intraepithelial neoplasia. Methods A total of 447 cervical cytology samples obtained from women who underwent colposcopy were examined. The cases were distributed as follows: (1) cervices without cervical intraepithelial neoplasia (CIN; n = 152); (2) cervices with a CIN grade of 1 (CIN 1; n = 147); and (3) cervices with a CIN grade of 2 or 3 (CIN 2/3; n = 148). The methylation pattern for a panel of 15 genes was analysed by quantitative methylation-specific PCR (qMSP) and compared between the groups (≤CIN 1 vs. CIN 2+). Results In the validation set, seven genes presented significantly different methylation profiles according to diagnosis, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), HIC1 (p = 0.028), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). Six genes showed a significant increase in the frequency of methylation in the presence of hr-HPV, namely, DAPK1 (p = 0.001), EPB41L3 (p = 0.001), hsa-miR-124-2 (p = 0.001), LMX1A (p = 0.001), SOX1 (p = 0.001), and TERT (p = 0.001). The methylation of the hsa-miR-124 gene showed sensitivity and specificity (86.7% and 61.3%, respectively) similar to that of the HPV test (91.3% and 50.0%, respectively). The independent factors associated with the diagnosis of CIN 2+ and the methylation of the hsa-miR-124-2 (OR = 5.1), SOX1 (OR = 2.8), TERT (OR = 2.2), and LMX1A (OR = 2.0) genes were a positive test for hr-HPV (odds ratio [OR] = 5.5). Conclusions Hypermethylation of the hsa-miR-124-2, SOX1, TERT, and LMX1A genes may be a promising biomarker for precursor lesions in cervical cancer regardless of the hr-HPV status.<br />São Paulo Research Foundation (Fundação de Amparo à Pesquisa do Estado de São Paulo–FAPESP) with the cooperation of the Coordination for the Improvement of Higher Education Personnel (Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq-PPSUS) (Protocol Nos. 12/51221-4, 14/50014-0, 14/24415-8, and 15/15065-6), the National Institute of Science and Technology (Instituto Nacional de Ciência e Tecnologia–INCT-HPV) (FAPESP Proc. No. 2008/578891 and CNPq Proc. No. 573799/2008-3), and CAPES (PROSUP/CAPES Protocol No. 1571815)<br />info:eu-repo/semantics/publishedVersion
- Subjects :
- 0301 basic medicine
Medicina Básica [Ciências Médicas]
Uterine Cervical Neoplasms
Gastroenterology
0302 clinical medicine
Cervical cytology
Papillomaviridae
Promoter Regions, Genetic
Telomerase
Early Detection of Cancer
Cervical cancer
Colposcopy
Intraepithelial neoplasia
biology
medicine.diagnostic_test
Obstetrics and Gynecology
Methylation
Middle Aged
3. Good health
Oncology
030220 oncology & carcinogenesis
Ciências Médicas::Medicina Básica
DNA methylation
Biomarker (medicine)
Female
Adult
Cervical cancer prevention
Human papillomavirus
medicine.medical_specialty
LIM-Homeodomain Proteins
Tumour suppressor genes
Cervical intraepithelial neoplasia
Sensitivity and Specificity
03 medical and health sciences
Internal medicine
Biomarkers, Tumor
medicine
Humans
Cervical Intraepithelial Neoplasia
Science & Technology
business.industry
SOXB1 Transcription Factors
Papillomavirus Infections
DNA Methylation
Uterine Cervical Dysplasia
MULHERES
medicine.disease
biology.organism_classification
MicroRNAs
030104 developmental biology
business
Transcription Factors
Subjects
Details
- ISSN :
- 00908258
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- Gynecologic Oncology
- Accession number :
- edsair.doi.dedup.....cf0184b5fc04ecec2ff38fa23227343e