Back to Search
Start Over
A Genetically Encoded Ratiometric pH Probe: Wavelength Regulation-Inspired Design of pH Indicators
- Source :
- ChemBioChem. 19:1288-1295
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Mutants of human cellular retinol-binding protein II (hCRBPII) were engineered to bind a julolidine retinal analogue for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, which affects the absorption and, thus, the emission of the protein/fluorophore complex. The ratio of emissions obtained at two excitation wavelengths that correspond to the absorption of the two forms of the protein/fluorophore complex, leads to a concentration-independent measure of pH.
- Subjects :
- Models, Molecular
Absorption (pharmacology)
Fluorophore
Protein Conformation
Biosensing Techniques
010402 general chemistry
01 natural sciences
Biochemistry
Fluorescence
Article
Ph probe
chemistry.chemical_compound
Humans
Molecular Biology
Julolidine
Fluorescent Dyes
010405 organic chemistry
Organic Chemistry
Retinol-Binding Proteins, Cellular
Protein engineering
Hydrogen-Ion Concentration
0104 chemical sciences
Transport protein
Wavelength
Spectrometry, Fluorescence
chemistry
Amino acid side chain
Mutagenesis, Site-Directed
Retinaldehyde
Biophysics
Molecular Medicine
Subjects
Details
- ISSN :
- 14394227
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- ChemBioChem
- Accession number :
- edsair.doi.dedup.....cef2386bdbd34bc8f6f0838f3cda8609