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Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging
- Source :
- Toxins, Toxins, Vol 8, Iss 4, p 85 (2016), Toxins; Volume 8; Issue 4; Pages: 85
- Publication Year :
- 2016
- Publisher :
- MDPI, 2016.
-
Abstract
- The venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to β-neurotoxins (β-NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main β-NTx of M. fulvius venom. β-NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%-78%; radiochemical purity ≥92%; and stability at 48 h ≥ 85%. The median lethal dose (LD50) and PLA₂ activity of bioconjugated β-NTx decreased 3 and 2.5 times, respectively, in comparison with native β-NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of β-NTx-DTPA-(67)Ga in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with (67)Ga-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of β-NTx-DTPA-(67)Ga remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of β-NTx-DTPA-(67)Ga. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.
- Subjects :
- 0301 basic medicine
radiolabeling
Gadolinium DTPA
Male
Biodistribution
Pathology
medicine.medical_specialty
food.ingredient
Single Photon Emission Computed Tomography Computed Tomography
Health, Toxicology and Mutagenesis
Neurotoxins
lcsh:Medicine
Venom
Toxicology
Median lethal dose
Article
Lethal Dose 50
Lymphatic System
03 medical and health sciences
Mice
0302 clinical medicine
food
medicine
Neurotoxin
Animals
Micrurus
Tissue Distribution
Elapidae
Rats, Wistar
Coral snake
Elapid Venoms
biology
lcsh:R
coral snake
Serum Albumin, Bovine
neurotoxin
biology.organism_classification
molecular imaging
030104 developmental biology
Lymphatic system
Polyclonal antibodies
030220 oncology & carcinogenesis
biology.protein
lymphatic absorption
human activities
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....ceefa84cca2bddc646ea62fd3a2fb9d5