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IL-6 protects pancreatic islet beta cells from pro-inflammatory cytokines-induced cell death and functional impairment in vitro and in vivo

Authors :
Gregory L. Szot
Chung Gyu Park
Duck Jong Han
Jung Sik Kim
S.C. Kim
Seung Eun Choi
Woong-Yang Park
Curie Ahn
Chang Yong Cha
Il Hee Yoon
Kyung Mi Choi
Jin-Young Shin
Kun Ho Yoon
Jae Young Kim
Eung Soo Hwang
Source :
Transplant Immunology. 13:43-53
Publication Year :
2004
Publisher :
Elsevier BV, 2004.

Abstract

Protection of pancreatic islet beta cells from pro-inflammatory cytokines-induced cell death and functional impairment is a key issue in developing therapeutic interventions of type 1 diabetes mellitus including islet transplantation. The effects of IL-6 on the protection of beta cells in vitro and in vivo were examined. Freshly isolated islets or MIN6 beta cells, when pre-incubated with IL-6, showed significantly higher viabilities measured by MTT assay and FACS analysis of PI stained cells against pro-apoptotic signaling delivered by IL-1beta, TNF-alpha and IFN-gamma. Insulin secretory function was also significantly protected in static culture with glucose and KCl stimulation. In vivo assessment using marginal mass syngeneic islet transplantation in mouse model revealed IL-6 conferred significantly better blood glucose control and graft survival rate over 50 days. Conclusively, IL-6 protects pancreatic islets or beta-cells from inflammatory cytokines-induced cell death and functional impairment both in vitro and in vivo. This strategy could be exploited in the clinical setting to maintain functional islet mass.

Details

ISSN :
09663274
Volume :
13
Database :
OpenAIRE
Journal :
Transplant Immunology
Accession number :
edsair.doi.dedup.....cece4ae2e9f4ed1237c2b58197c52f6a
Full Text :
https://doi.org/10.1016/j.trim.2004.04.001