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Comparative Transcriptome Analysis Reveals Different Host Cell Responses to Acute and Persistent Foot-and-Mouth Disease Virus Infection
- Source :
- Virol Sin
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Foot-and-mouth disease virus (FMDV) rapidly causes cytopathic effects in susceptible cells. Incomplete viral clearance during the acute infection leads to persistent infection. The relationship between host gene expression and the persistent infection remains unclear. In this study, we analyzed the transcriptome profiles of BHK-21 cells acutely and persistently infected with FMDV to identify differences in gene expression. GO and KEGG enrichment analyses showed that the 8,378 differentially expressed genes were significantly enriched in categories including metabolism, biosynthesis, ribosome function, and endocytosis. In persistently infected BHK-21 cells, ribosome- and translation-related genes were significantly down-regulated. There were more differentially expressed immune-related genes during persistent infection than during acute infection. Two hundred and seventy-four genes were differentially expressed in both acutely and persistently infected BHK-21 cells. Among these genes, heat shock protein family B member 1 (Hspb1) knockdown significantly inhibited FMDV replication. Our research provides a basis for further research to understand the mechanisms of persistent FMDV infection including the genes involved in FMDV replication. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12250-019-00155-8) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
viruses
030106 microbiology
Immunology
Biology
Kidney
Virus Replication
Virus
Cell Line
Transcriptome
03 medical and health sciences
Cricetinae
Virology
Heat shock protein
Gene expression
Animals
KEGG
Gene
Immunity, Cellular
Gene knockdown
Gene Expression Profiling
biology.organism_classification
030104 developmental biology
Foot-and-Mouth Disease Virus
Foot-and-Mouth Disease
Acute Disease
Host-Pathogen Interactions
RNA, Viral
Molecular Medicine
Foot-and-mouth disease virus
Research Article
Subjects
Details
- ISSN :
- 1995820X and 16740769
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Virologica Sinica
- Accession number :
- edsair.doi.dedup.....ceb340d64d4d809ad10a4052bda304ea