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Pooling samples : the key to sensitive, specific and cost-effective genetic diagnosis of Chlamydia trachomatis in low-resource countries
- Publication Year :
- 2007
- Publisher :
- Institutionen för medicinska vetenskaper, 2007.
-
Abstract
- The aims of this study were to compare the performance characteristics and cost-effectiveness of pooling endocervical samples for screening and diagnosis of Chlamydia trachomatis, and to investigate the prevalence of C. trachomatis infection in women in Leningrad Oblast, Russia. A total of 1500 endocervical samples were tested individually and when pooled in groups of 5 and 10 samples, respectively. A previously evaluated in-house diagnostic polymerase chain reaction (PCR) assay was utilized. The sensitivity and specificity of the PCR were not affected by either pooling strategy. The estimated prevalence of genital C. trachomatis infection was 6.6%, 6.1% and 6.0% based on individually tested samples, and pools of 5 and 10, respectively. For diagnosis of individual samples, the pooling strategies resulted in cost savings of 53.3% (5 samples per pool) and 44.0% (10 samples per pool). Pooling samples for PCR detection of C. trachomatis is an accurate and cost-saving approach for diagnosis and large-scale prevalence studies in St Petersburg, Russia.
- Subjects :
- Pooling
Veterinary medicine
Medicin och hälsovetenskap
Low resource
Sample (statistics)
Chlamydia trachomatis
Cervix Uteri
Dermatology
medicine.disease_cause
Polymerase Chain Reaction
Sensitivity and Specificity
Medical and Health Sciences
law.invention
Russia
law
Humans
Medicine
Chlamydiaceae
NAAT
Developing Countries
Diagnostics
Polymerase chain reaction
Vaginal Smears
biology
business.industry
General Medicine
Chlamydia Infections
biology.organism_classification
PCR
Chlamydiales
Female
business
Genetic diagnosis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....ceb32b5c17309537f46cd3f17509979e