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Heshouwu (Polygonum multiflorum Thunb.) ethanol extract suppresses pre-adipocytes differentiation in 3T3-L1 cells and adiposity in obese mice
- Source :
- Biomedicine & Pharmacotherapy. 106:355-362
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- This study investigated whether Heshouwu (Polygonum multiflorum Thunb.) root ethanol extract (PME) has anti-obesity activity using 3T3-L1 cells and high-fat diet (HFD)-induced obese mice. Treatment with PME (5 and 10 μg/mL) dose-dependently suppressed 3T3-L1 pre-adipocyte differentiation to adipocytes and cellular triglyceride contents. In addition, PME inhibited mRNA and protein expression of adipogenic transcription factors such as CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ), which led to down-regulation of fatty acid synthase gene expression. After feeding mice PME (0.05%) with HFD for 12 weeks, their visceral fat mass, size and body weight were significantly reduced compared with the HFD group. Furthermore, PME supplementation significantly up-regulated the PPARα, CPT1, CPT2, UCP1 and HSL mRNA levels compared with the HFD group, whereas it down-regulated expression of the PPARγ and DGAT2 genes. Finally, HFD increased serum leptin, insulin, glucose and insulin and glucose levels; however, PME reversed these changes. These results demonstrated that PME might relieve obesity that occurs via inhibition of adipogenesis and lipogenesis as well as through lipolysis and fatty acid oxidation in 3T3-L1 cells and HFD-induced obese mice.
- Subjects :
- Blood Glucose
Leptin
Male
0301 basic medicine
Time Factors
medicine.medical_treatment
Mice
chemistry.chemical_compound
0302 clinical medicine
Adipocytes
Insulin
Receptor
Beta oxidation
Adiposity
Adipogenesis
Chemistry
Fatty Acids
food and beverages
General Medicine
Peroxisome
Lipogenesis
Fallopia multiflora
Oxidation-Reduction
medicine.medical_specialty
Lipolysis
030209 endocrinology & metabolism
Intra-Abdominal Fat
03 medical and health sciences
3T3-L1 Cells
Internal medicine
medicine
Animals
Obesity
Triglycerides
Pharmacology
Plants, Medicinal
Dose-Response Relationship, Drug
Ethanol
Triglyceride
Plant Extracts
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Endocrinology
Gene Expression Regulation
Solvents
Anti-Obesity Agents
Phytotherapy
Subjects
Details
- ISSN :
- 07533322
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- Biomedicine & Pharmacotherapy
- Accession number :
- edsair.doi.dedup.....ceb17777db6f2af8b228149710b050f8