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Functional characterization and evaluation of protective efficacy of EA752-862 monoclonal antibody against B. anthracis vegetative cell and spores
- Source :
- Medical microbiology and immunology. 209(2)
- Publication Year :
- 2019
-
Abstract
- The most promising means of controlling anthrax, a lethal zoonotic disease during the early infection stages, entail restricting the resilient infectious form, i.e., the spores from proliferating to replicating bacilli in the host. The extractible antigen (EA1), a major S-layer protein present on the vegetative cells and spores of Bacillus anthracis, is highly immunogenic and protects mice against lethal challenge upon immunization. In the present study, mice were immunized with r-EA1C, the C terminal crystallization domain of EA1, to generate a neutralizing monoclonal antibody EA752–862, that was evaluated for its anti-spore and anti-bacterial properties. The monoclonal antibody EA752–862 had a minimum inhibitory concentration of 0.08 mg/ml, was bactericidal at a concentration of 0.1 mg/ml and resulted in 100% survival of mice against challenge with B. anthracis vegetative cells. Bacterial cell lysis as observed by scanning electron microscopy and nucleic acid leakage assay could be attributed as a possible mechanism for the bactericidal property. The association of mAb EA752–862 with spores inhibits their subsequent germination to vegetative cells in vitro, enhances phagocytosis of the spores and killing of the vegetative cells within the macrophage, and subsequently resulted in 90% survival of mice upon B. anthracis Ames spore challenge. Therefore, owing to its anti-spore and bactericidal properties, the present study demonstrates mAb EA752–862 as an efficient neutralizing antibody that hinders the establishment of early infection before massive multiplication and toxin release takes place.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
medicine.drug_class
Phagocytosis
030106 microbiology
Immunology
Microbial Sensitivity Tests
Monoclonal antibody
medicine.disease_cause
Microbiology
Anthrax
03 medical and health sciences
Minimum inhibitory concentration
Mice
Antigen
medicine
Immunology and Allergy
Animals
Neutralizing antibody
Spores, Bacterial
Antigens, Bacterial
Mice, Inbred BALB C
Binding Sites
biology
Chemistry
Toxin
Macrophages
fungi
Antibodies, Monoclonal
General Medicine
biology.organism_classification
Antibodies, Bacterial
Antibodies, Neutralizing
Spore
Bacillus anthracis
Anti-Bacterial Agents
030104 developmental biology
biology.protein
Microscopy, Electron, Scanning
Female
Immunization
Subjects
Details
- ISSN :
- 14321831
- Volume :
- 209
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Medical microbiology and immunology
- Accession number :
- edsair.doi.dedup.....ceb0b167a4dfe8858ebef95e63fd4b74