Preparation and assay of urogastrone, an inhibitor of gastric acid secretion

Summary 1 Urogastrone, an acid gastric secretion inhibitor extracted from human urine, was assayed in the anaesthetized rat by circulating through its stomach a succinic-propionic buffer and recording electrometrically the secretion of acid in response to carbachol, and inhibition of carbachol secretion by urogastrone. 2 Quantitative assay procedures are described requiring the administration of only one or two doses of urogastrone to each rat. 3 An extraction procedure for urogastrone is described based on the use of zeocarb, a cationic polystyrene resin. 4 The most purified samples contained 40–80 times the amount/milligram which was just detectable by the assay method.