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Use of FTIR spectroscopy to distinguish between capsular types and capsular quantities inStreptococcus pneumoniae
- Source :
- Biopolymers. 83:434-442
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- Fourier transform infrared (FTIR) spectroscopy has shown remarkable ability in distinguishing between bacterial species and identifying bacterial colony structures, when used in tandem with methods such as cluster analysis, principal component analysis, or linear discriminant analysis. The present work was aimed to evaluate the potential of FTIR–microscopy (FTIR-MSP) to distinguish between different serotypes and capsular quantities of Streptococcus pneumoniae. In general, the results obtained have consistently proven that the spectral information at the region 900–1185 cm−1 was sufficient to distinguish between various pneumococcal serotypes. Moreover, the method was able to differentiate between S. pneumoniae phase variants on the basis of their relative carbohydrate content. The unsupervised cluster analysis of the samples showed differences, not only in the carbohydrate content, but also in the region 1350–1480 cm−1, which is dominated by absorptions due to lipids and phospholipids. This approach proved to be useful for the distinction between S. pneumoniae serotypes and between phase variants, which were shown to acquire different pathogenic capacity. © 2006 Wiley Periodicals, Inc. Biopolymers 83:434–442, 2006 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
- Subjects :
- Pneumococcal serotypes
Carbohydrate content
Chemistry
Organic Chemistry
Biophysics
General Medicine
Computational biology
medicine.disease_cause
Biochemistry
Microbiology
Biomaterials
Streptococcus pneumoniae
Spectroscopy, Fourier Transform Infrared
Principal component analysis
medicine
Cluster Analysis
Serotyping
Fourier transform infrared spectroscopy
Bacterial Capsules
Bacterial colony
Subjects
Details
- ISSN :
- 10970282 and 00063525
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Biopolymers
- Accession number :
- edsair.doi.dedup.....ce985d16ed6ecfa48897fe7c88412186