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Accurate light microscopic diagnosis of South-East Asian ovalocytosis
- Source :
- International Journal of Laboratory Hematology. 40:655-662
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Introduction: Southeast Asian ovalocytosis (SAO) is a common inherited red blood cell polymorphism in Southeast Asian and Melanesian populations, coinciding with areas of malaria endemicity. Validation of light microscopy as a diagnostic alternative to molecular genotyping may allow for its cost-effective use either prospectively or retrospectively by analysis of archived blood smears. Methods: We assessed light microscopic diagnosis of SAO compared to standard PCR genotyping. Three trained microscopists, each assessed the same 971 Giemsa-stained thin blood films for which SAO genotypic confirmation was available by PCR. Generalized mixed modeling was used to estimate the sensitivity, specificity, positive predictive value, and negative predictive value of light microscopy versus “gold-standard” PCR. Results: Among red cell morphologic parameters evaluated, knizocytes, rather than ovalocytic morphology, proved the strongest predictor of SAO status (odds ratio [OR] =19.2; 95% confidence interval [95% CI] = 14.6-25.3; P = Conclusion: Light microscopic diagnosis of SAO by knizocyte visual cue performed comparable to time-consuming and costlier molecular methods, but requires specific training that includes successful differentiation of knizocytes from stomatocytes.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Clinical Biochemistry
Erythrocytes, Abnormal
Southeast asian
Polymerase Chain Reaction
Gastroenterology
Article
03 medical and health sciences
0302 clinical medicine
Internal medicine
parasitic diseases
Genotype
medicine
Humans
Microscopist
Genotyping
Asia, Southeastern
Microscopy
business.industry
Biochemistry (medical)
Elliptocytosis, Hereditary
Hematology
General Medicine
Odds ratio
medicine.disease
Southeast Asian ovalocytosis
Confidence interval
030104 developmental biology
Ovalocytosis
030220 oncology & carcinogenesis
Female
business
Subjects
Details
- ISSN :
- 17515521
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- International Journal of Laboratory Hematology
- Accession number :
- edsair.doi.dedup.....ce9535768c16b32909471206861e5f06