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Polo-like kinase 1 phosphorylation of G2 and S-phase-expressed 1 protein is essential for p53 inactivation during G2 checkpoint recovery
- Source :
- EMBO reports. 11(8)
- Publication Year :
- 2010
-
Abstract
- In response to G2 DNA damage, the p53 pathway is activated to lead to cell-cycle arrest, but how p53 is eliminated during the subsequent recovery process is poorly understood. It has been established that Polo-like kinase 1 (Plk1) controls G2 DNA-damage recovery. However, whether Plk1 activity contributes to p53 inactivation during this process is unknown. In this study, we show that G2 and S-phase-expressed 1 (GTSE1) protein, a negative regulator of p53, is required for G2 checkpoint recovery and that Plk1 phosphorylation of GTSE1 at Ser 435 promotes its nuclear localization, and thus shuttles p53 out of the nucleus to lead to its degradation during the recovery.
- Subjects :
- G2 Phase
DNA damage
Recombinant Fusion Proteins
Cell Cycle Proteins
Polo-like kinase
Biology
Protein Serine-Threonine Kinases
Biochemistry
PLK1
Cell Line
Proto-Oncogene Proteins
Genetics
Humans
Phosphorylation
Cell Cycle Protein
Molecular Biology
Kinase
Scientific Reports
G2-M DNA damage checkpoint
Cell biology
RNA Interference
Tumor Suppressor Protein p53
Microtubule-Associated Proteins
Nuclear localization sequence
DNA Damage
Subjects
Details
- ISSN :
- 14693178
- Volume :
- 11
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- EMBO reports
- Accession number :
- edsair.doi.dedup.....ce82bb01f0e1bb5837e140a92145e94b